Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2'-fucosyllactose | substrate inhibition above 1 mM | Homo sapiens | |
lacto-N-fucopentaose I | substrate inhibition above 2 mM | Homo sapiens | |
UDP | - |
Homo sapiens | |
UDP-galactose | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
UDP-GalNAc | - |
Homo sapiens | |
0.27 | - |
2'-fucosyllactose | - |
Homo sapiens | |
0.35 | - |
lacto-N-fucopentaose I | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 35000, SDS-PAGE with and without 2-mercaptoethanol | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
blood group A | - |
Purification (Comment) | Organism |
---|---|
blood group A | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood plasma | A | Homo sapiens | - |
Storage Stability | Organism |
---|---|
-20°C, rapid loss of activity | Homo sapiens |
-80°C, 20% loss of activity after 1 year | Homo sapiens |
4°C, 0.05 M Tris-HCl, pH 7.4, 2 mM MnCl2, 1 mM EDTA, 0.01% w/v Triton X-100, 0.2 M NaCl, 1 mM dithiothreitol, 0.03% w/v NaN3, 10% loss of activity per month | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme also catalyzes the transfer of galactose in alpha-linkage to 2'-fucosyllactose, the transfer rate of galactose is much lower than that of N-acetylgalactosamine | Homo sapiens | ? | - |
? | |
UDP-N-acetyl-D-galactosamine + 2'-fucosyllactose | - |
Homo sapiens | UDP + N-acetyl-alpha-D-galactosaminyl-2'-fucosyllactose | - |
? | |
UDP-N-acetyl-D-galactosamine + glycoprotein alpha-L-fucosyl-1,2-D-galactose | - |
Homo sapiens | UDP + glycoprotein N-acetyl-alpha-D-galactosaminyl-1,3-[alpha-L-fucosyl-1,2]-D-galactose | - |
? | |
UDP-N-acetyl-D-galactosamine + lacto-N-fucopentaose I | i.e. Fucalpha(1-2)Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc | Homo sapiens | UDP + N-acetyl-alpha-D-galactosaminyl-1,3-[lacto-N-fucopentaose I] | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, SDS-PAGE with and without 2-mercaptoethanol | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
30 min, 5% loss of activity | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
- |
Homo sapiens |