Application | Comment | Organism |
---|---|---|
biotechnology | arbutin as a safe hydroquinone derivative is one of most important skin-whitening ingredients including beta-arbutin and alpha-arbutin. The batch-feeding whole-cell biocatalysis by Amy-1 is a promising technology for alpha-arbutin production with enhanced yield and molar conversion rate | Xanthomonas campestris pv. campestris |
synthesis | the batch-feeding whole-cell biocatalysis by Amy-1 is a promising technology for alpha-arbutin production with enhanced yield and molar conversion rate | Xanthomonas campestris pv. campestris |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Xanthomonas campestris pv. campestris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 1.0 mM, 12% inhibition | Xanthomonas campestris pv. campestris | |
Cu2+ | 1.0 mM, complete inhibition | Xanthomonas campestris pv. campestris | |
Mn2+ | 1.0 mM, 50% inhibition | Xanthomonas campestris pv. campestris | |
Ni2+ | 1.0 mM, 30% inhibition | Xanthomonas campestris pv. campestris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.58 | - |
hydroquinone | pH 7.0, 30°C | Xanthomonas campestris pv. campestris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 1.0 mM, 40% activation | Xanthomonas campestris pv. campestris | |
K+ | 1.0 mM, 10% activation | Xanthomonas campestris pv. campestris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas campestris pv. campestris | A0A0H2X5U3 | - |
- |
Xanthomonas campestris pv. campestris 8004 | A0A0H2X5U3 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Xanthomonas campestris pv. campestris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + hydroquinone | - |
Xanthomonas campestris pv. campestris | D-fructose + alpha-arbutin | - |
? | |
sucrose + hydroquinone | - |
Xanthomonas campestris pv. campestris 8004 | D-fructose + alpha-arbutin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Amy-1 | - |
Xanthomonas campestris pv. campestris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Xanthomonas campestris pv. campestris |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 40 | 20°C: about 60% of maximal activity, 40°C: about 70% of maximal activity | Xanthomonas campestris pv. campestris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
high stability, maintaining 83% activity after 2 h | Xanthomonas campestris pv. campestris |
50 | - |
complete loss of catalytic activity after 30 min | Xanthomonas campestris pv. campestris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
23.9 | - |
hydroquinone | pH 7.0, 30°C | Xanthomonas campestris pv. campestris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Xanthomonas campestris pv. campestris |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.5 | pH 5.0: 65% of maximal activity, pH 8.0: 45% of maximal activity | Xanthomonas campestris pv. campestris |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | - |
hydroquinone | pH 7.0, 30°C | Xanthomonas campestris pv. campestris |