Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.4 extracted from

  • Zhu, X.; Tian, Y.; Xu, W.; Bai, Y.; Zhang, T.; Mu, W.
    Biochemical characterization of a highly thermostable amylosucrase from Truepera radiovictrix DSM 17093 (2018), Int. J. Biol. Macromol., 116, 744-752 .
    View publication on PubMed
Search for more literature for 2.4.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Truepera radiovictrix

Organism

Organism UniProt Comment Textmining
Truepera radiovictrix D7CVD0
-
-
Truepera radiovictrix DSM 17093 D7CVD0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose when the conversion of 100 mM sucrose is catalyzed at 35°C, 45°C, and 55°C for 24 h, the enzyme produces alpha-(1,4) glucans with average degrees of polymerisation of 59, 45, and 37, respectively Truepera radiovictrix alpha-(1,4) glucan
-
 ?
sucrose when the conversion of 100 mM sucrose is catalyzed at 35°C, 45°C, and 55°C for 24 h, the enzyme produces alpha-(1,4) glucans with average degrees of polymerisation of 59, 45, and 37, respectively Truepera radiovictrix DSM 17093 alpha-(1,4) glucan
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Truepera radiovictrix

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 total and transglucosylation activity Truepera radiovictrix
50
-
 hydrolysis activity Truepera radiovictrix

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 displays more than 90% of its original activity after incubation at 55°C for 5 h Truepera radiovictrix
60
-
 retains 73% of its original activity after incubation for 3 h Truepera radiovictrix
65
-
 the enzyme keeps half of its original activity after incubation for 1.22 h Truepera radiovictrix
69.92
-
 melting temperature Truepera radiovictrix

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 hydrolysis activity Truepera radiovictrix
7.5
-
 total and transglucosylation activities Truepera radiovictrix