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Literature summary for 2.4.1.4 extracted from

  • Huang, X.F.; Nazarian-Firouzabadi, F.; Vincken, J.P.; Ji, Q.; Visser, R.G.; Trindade, L.M.
    Expression of an amylosucrase gene in potato results in larger starch granules with novel properties (2014), Planta, 240, 409-421.
    View publication on PubMed

Application

Application Comment Organism
biotechnology the enzyme fused to a starch-binding domain (SBD) is introduced in two potato genetic backgrounds to synthesize starch granules with altered composition, and thereby to broaden starch applications. The modified larger starches not only have great benefit to the potato starch industry by reducing losses during starch isolation, but also have an advantage in many food applications such as frozen food due to its extremely high freeze-thaw stability. Modified starches show a higher digestibility after alpha-amylase treatment Neisseria polysaccharea

Cloned(Commentary)

Cloned (Comment) Organism
gene ams, recombinant expression as starch-binding domain-fusion protein in Solanum tuberosum plants, cv. Kardal and amf, using the Agrobacterium tumefaciens transformation method Neisseria polysaccharea

Protein Variants

Protein Variants Comment Organism
additional information amylosucrase from Neisseria polysaccharea is fused to a starch-binding domain (SBD) of cyclodextrin glycosyltransferase from Bacillus circulans, expression of the amylosucrase-SBD and SBD-amylosucrase fusion proteins in the amylose-containing (cv. Kardal) and amylose-free (amf) Solanum tuberosum plants, respectively. Expression of SBD-amylosucrase fusion protein in the amylose-containing potato results in starch granules with a rough surface, a twofold increase in median granule size, and altered physico-chemical properties including improved freeze-thaw stability, higher end viscosity, and better enzymatic digestibility. These effects are possibly a result of the physical interaction between amylosucrase and starch granules Neisseria polysaccharea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72466
-
x * 72466, sequence calculation, excluding the transit peptide, x * 85000, about, recombinant SBD-fusion enzyme, SDS-PAGE Neisseria polysaccharea
85000
-
x * 72466, sequence calculation, excluding the transit peptide, x * 85000, about, recombinant SBD-fusion enzyme, SDS-PAGE Neisseria polysaccharea

Organism

Organism UniProt Comment Textmining
Neisseria polysaccharea Q9ZEU2
-
-

Subunits

Subunits Comment Organism
? x * 72466, sequence calculation, excluding the transit peptide, x * 85000, about, recombinant SBD-fusion enzyme, SDS-PAGE Neisseria polysaccharea