Cloned (Comment) | Organism |
---|---|
DNA library construction and DNA sequence determination and analysis, expression of the His-tagged enzyme in Escherichia coli | Neisseria polysaccharea |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of amylosucrase variants that terminate catalysis of acceptor elongation at the di- or trisaccharide stage, product patterns formed by wild-type enzyme and selected genetic variants in the presence of sucrose as the sole substrate, overview | Neisseria polysaccharea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neisseria polysaccharea | Q9ZEU2 | strain ATCC 43768 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli | Neisseria polysaccharea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | product patterns formed by wild-type enzyme and selected genetic variants in the presence of sucrose as the sole substrate, overview | Neisseria polysaccharea | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Neisseria polysaccharea |