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Literature summary for 2.4.1.4 extracted from

  • Schneider, J.; Fricke, C.; Overwin, H.; Hofmann, B.; Hofer, B.
    Generation of amylosucrase variants that terminate catalysis of acceptor elongation at the di- or trisaccharide stage (2009), Appl. Environ. Microbiol., 75, 7453-7460.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
DNA library construction and DNA sequence determination and analysis, expression of the His-tagged enzyme in Escherichia coli Neisseria polysaccharea

Protein Variants

Protein Variants Comment Organism
additional information generation of amylosucrase variants that terminate catalysis of acceptor elongation at the di- or trisaccharide stage, product patterns formed by wild-type enzyme and selected genetic variants in the presence of sucrose as the sole substrate, overview Neisseria polysaccharea

Organism

Organism UniProt Comment Textmining
Neisseria polysaccharea Q9ZEU2 strain ATCC 43768
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Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli Neisseria polysaccharea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information product patterns formed by wild-type enzyme and selected genetic variants in the presence of sucrose as the sole substrate, overview Neisseria polysaccharea ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at room temperature Neisseria polysaccharea