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Literature summary for 2.4.1.4 extracted from

  • Skov, L.K.; Mirza, O.; Henriksen, A.; de Montalk, G.P.; Remaud-Simeon, M.; Sarcabal, P.; Willemot, R.M.; Monsan, P.; Gajhede, M.
    Amylosucrase, a glucan-synthesizing enzyme from the alpha-amylase family (2001), J. Biol. Chem., 276, 25273-25278.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
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Neisseria polysaccharea

Crystallization (Commentary)

Crystallization (Comment) Organism
recombinant enzyme, equal amounts of 4 mg/ml enzyme in 150 mM NaCl, 50 mM Tris-HCl, pH 7.0, 1 mM EDTA and 1 mM dithiothreitol and reservoir solution consisting of 30% polyethylene glycol 6000 and 100 mM HEPES, pH 7.0, crystal structure at 1.4 A resolution Neisseria polysaccharea

Organism

Organism UniProt Comment Textmining
Neisseria polysaccharea Q9ZEU2
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-

Purification (Commentary)

Purification (Comment) Organism
-
Neisseria polysaccharea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose enzyme catalyzes both sucrose hydrolysis and oligosaccharide and polymer synthesis in the absence of an activator polymer Neisseria polysaccharea glucose + maltose + maltotriose + turanose + insoluble polymer with 10 mM sucrose as the sole substrate, 30% glucose, 29% maltose, 18% maltotriose, 11% turanose and 12% insoluble polymer respectively ?