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Literature summary for 2.4.1.4 extracted from

  • De Montalk, G.P.; Remaud-Simeon, M.; Willemot, R.M.; Planchot, V.; Monsan, P.
    Sequence analysis of the gene encoding amylosucrase from Neisseria polysaccharea and characterization of the recombinant enzyme (1999), J. Bacteriol., 181, 375-381.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Neisseria polysaccharea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
-
Neisseria polysaccharea
70000
-
native PAGE Neisseria polysaccharea

Organism

Organism UniProt Comment Textmining
Neisseria polysaccharea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Neisseria polysaccharea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + (1,4-alpha-D-glucosyl)n
-
Neisseria polysaccharea D-fructose + (1,4-alpha-D-glucosyl)n+1 recombinant enzyme linearly elongates some branched chains of glycogen to an average degree of polymerization of 75 ?

Subunits

Subunits Comment Organism
monomer 1 * 70000, SDS-PAGE Neisseria polysaccharea

pI Value

Organism Comment pI Value Maximum pI Value
Neisseria polysaccharea
-
-
4.9