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Literature summary for 2.4.1.389 extracted from
- Discovery of solabiose phosphorylase and its application for enzymatic synthesis of solabiose from sucrose and lactose (2022), Sci. Rep., 12, 259 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | production of solabiose from lactose and sucrose. Lactose is hydrolyzed to D-galactose and D-glucose by beta-galactosidase. Phosphorolysis of sucrose and synthesis of solabiose are then coupled by adding sucrose, sucrose phosphorylase, and enzyme PBOR_28850 to the reaction mixture. Using 210 mmol lactose and 280 mmol sucrose, 207 mmol of solabiose is produced. Yeast treatment degrades the remaining monosaccharides and sucrose without reducing solabiose. Solabiose with a purity of 93.7% is obtained without any chromatographic procedures | Paenibacillus borealis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.53 | - |
phosphate | pH 6.5, 30°C | Paenibacillus borealis |  |
| 2.26 | - |
solabiose | pH 6.5, 30°C | Paenibacillus borealis | |
| 4.27 | - |
alpha-D-glucose 1-phosphate | pH 6.5, 30°C | Paenibacillus borealis | |
| 7.35 | - |
D-galactose | pH 6.5, 30°C | Paenibacillus borealis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactose + alpha-D-glucose 1-phosphate | Paenibacillus borealis | - |
solabiose + phosphate | - |
r | |
| solabiose + phosphate | Paenibacillus borealis | - |
D-galactose + alpha-D-glucose 1-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paenibacillus borealis | A0A089LI24 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactose + alpha-D-glucose 1-phosphate | - |
Paenibacillus borealis | solabiose + phosphate | - |
r | |
| solabiose + phosphate | - |
Paenibacillus borealis | D-galactose + alpha-D-glucose 1-phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 82000, SDS-PAGE, x * 85900, calculated from sequence | Paenibacillus borealis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PBOR_28850 | - |
Paenibacillus borealis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Paenibacillus borealis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
more than 85% of the original activity below | Paenibacillus borealis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.23 | - |
D-galactose | pH 6.5, 30°C | Paenibacillus borealis | |
| 9.14 | - |
solabiose | pH 6.5, 30°C | Paenibacillus borealis | |
| 14.5 | - |
alpha-D-glucose 1-phosphate | pH 6.5, 30°C | Paenibacillus borealis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
phosphorolysis of solabiose | Paenibacillus borealis |
| 7.8 | - |
synthesis of solabiose | Paenibacillus borealis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | enzyme belongs to glycoside hydrolase family 94 | Paenibacillus borealis |
| metabolism | sequential bi-bi mechanism involving the formation of a ternary complex | Paenibacillus borealis |
| physiological function | enzyme specifically utilizes D-galactose as an acceptor and produces solabiose, i.e. beta-D-Glcp-(1->3)-D-Gal | Paenibacillus borealis |
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