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Literature summary for 2.4.1.384 extracted from
- Mutational analyses for product specificity of YjiC towards alpha-mangostin mono-glucoside (2018), Enzyme Microb. Technol., 118, 76-82 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H298A | the mutant exhibits a regiospecificity towards glycosylated product alpha-mangostin 3-O-beta-D-glucopyranoside from alpha-mangostin while the production pattern of alpha-mangostin 3,6-di-O-beta-D-glucopyranoside decreases to 63% yields compared to the wild type enzyme. The mutant exhibits 244% increase in alpha-mangostin 3-O-beta-D-glucopyranoside production yield and also 281% increase in yield of alpha-mangostin 6-O-beta-D-glucopyranoside compared with wild type enzyme | Bacillus licheniformis |
| H298C | the mutant exhibits a regiospecificity towards glycosylated product alpha-mangostin 3-O-beta-D-glucopyranoside from alpha-mangostin while the production pattern of alpha-mangostin 3,6-di-O-beta-D-glucopyranoside decreases to 95% yields compared to the wild type enzyme. The mutant exhibits 186% increase in alpha-mangostin 3-O-beta-D-glucopyranoside production yield and also 251% increase in yield of alpha-mangostin 6-O-beta-D-glucopyranoside compared with wild type enzyme | Bacillus licheniformis |
| H298S | the mutant exhibits a regiospecificity towards glycosylated product alpha-mangostin 3-O-beta-D-glucopyranoside from alpha-mangostin while the production pattern of alpha-mangostin 3,6-di-O-beta-D-glucopyranoside decreases to 85% yields compared to the wild type enzyme. The mutant exhibits 251% increase in alpha-mangostin 3-O-beta-D-glucopyranoside production yield and also 279% increase in yield of alpha-mangostin 6-O-beta-D-glucopyranoside compared with wild type enzyme | Bacillus licheniformis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| UDP | increase of UDP leads to the inhibition of enzyme activity | Bacillus licheniformis |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 10 mM used in assay conditions | Bacillus licheniformis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus licheniformis | - |
- |
- |
| Bacillus licheniformis DSM-13 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + 3-hydroxyflavone | - |
Bacillus licheniformis | ? + UDP | - |
? | |
| UDP-alpha-D-glucose + 3-hydroxyflavone | - |
Bacillus licheniformis DSM-13 | ? + UDP | - |
? | |
| UDP-alpha-D-glucose + alpha-mangostin | - |
Bacillus licheniformis | alpha-mangostin 3,6-di-O-beta-D-glucopyranoside + alpha-mangostin 3-O-beta-D-glucopyranoside + alpha-mangostin 6-O-beta-D-glucopyranoside + UDP | - |
? | |
| UDP-alpha-D-glucose + alpha-mangostin | - |
Bacillus licheniformis DSM-13 | alpha-mangostin 3,6-di-O-beta-D-glucopyranoside + alpha-mangostin 3-O-beta-D-glucopyranoside + alpha-mangostin 6-O-beta-D-glucopyranoside + UDP | - |
? | |
| UDP-alpha-D-glucose + apigenin | - |
Bacillus licheniformis | ? + UDP | - |
? | |
| UDP-alpha-D-glucose + apigenin | - |
Bacillus licheniformis DSM-13 | ? + UDP | - |
? | |
| UDP-alpha-D-glucose + chrysin | - |
Bacillus licheniformis | ? + UDP | - |
? | |
| UDP-alpha-D-glucose + chrysin | - |
Bacillus licheniformis DSM-13 | ? + UDP | - |
? | |
| UDP-alpha-D-glucose + genistein | - |
Bacillus licheniformis | ? + UDP | - |
? | |
| UDP-alpha-D-glucose + genistein | - |
Bacillus licheniformis DSM-13 | ? + UDP | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| YjiC | - |
Bacillus licheniformis |
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