Literature summary for 2.4.1.373 extracted from

Malbert, Y.; Moulis, C.; Brison, Y.; Morel, S.; Andre, I.; Remaud-Simeon, M.
Engineering a branching sucrase for flavonoid glucoside diversification (2018), Sci. Rep., 8, 15153 .

Search for more literature for 2.4.1.373

Application

Application	Comment	Organism
synthesis	use of enzyme to generate a platform for flavonoid glucosylation. Engineered variants show remarkable ability for luteolin, morin and naringenin glucosylation with conversion ranging from 30% to 90%	Leuconostoc mesenteroides

Protein Variants

Protein Variants	Comment	Organism
W2135F	mutant displays 3-4fold improved activity with naringenin	Leuconostoc mesenteroides
W2135G	mutant displays 3-4fold improved activity with naringenin	Leuconostoc mesenteroides
W2135G	mutant synthesizes four times more morin glucoside than the wild-type	Leuconostoc mesenteroides
W2135I/F2136Y	mutant displays 3-4fold improved activity with naringenin, about 60% conversion. Mutant converts nearly 3-times more naringenin than luteolin (59% and 17%, conversion, respectively)	Leuconostoc mesenteroides
W2135L-F2136L	mutant significantly synthesizes quercetin di-glucoside	Leuconostoc mesenteroides
W2135V	mutant displays 3-4fold improved activity with naringenin	Leuconostoc mesenteroides

Organism

Organism	UniProt	Comment	Textmining
Leuconostoc mesenteroides	Q8G9Q2	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sucrose + luteolin	-	Leuconostoc mesenteroides	D-fructose + luteolin glucoside	-	?
sucrose + morin	-	Leuconostoc mesenteroides	D-fructose + morin glucoside	one mono-glucoside, three di-glucosides and one tri-glucoside	?
sucrose + naringenin	-	Leuconostoc mesenteroides	D-fructose + naringenin glucoside	one naringenin mono-glucoside, two di-glucosides and one tri-glucoside	?
sucrose + quercetin	-	Leuconostoc mesenteroides	D-fructose + quercetin glucoside	-	?

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Release 2023.1 (January 2023)