Activating Compound | Comment | Organism | Structure |
---|---|---|---|
alpha-L-Fuc-(1,2)-beta-D-3-deoxy-Gal-O-octyl | i.e. 3DD, an acceptor substrate analogue, accelerates enzymatic hydrolysis of UDP-Gal | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli strain BL21 | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates, GTB requires divalent metal ions for full activity | Homo sapiens | |
Mn2+ | activates 9fold at 24°C, GTB requires divalent metal ions for full activity | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-galactose + alpha-L-fucosyl-(1,2)-D-galactose | Homo sapiens | i.e. H disaccharide | UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl(1,2)]-D-galactose | - |
? | |
UDP-galactose + blood group antigen | Homo sapiens | GTB catalyzes the transfer of galactose from UDP-Gal to the C3 position of the terminal galactose of H antigen acceptors | UDP + alpha-D-galactosyl-blood group antigen | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli by cation exchange chromatograophy, UDP-hexanolamine affinity chromatography, and dialysis | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-galactose + alpha-L-fucosyl-(1,2)-D-galactose | i.e. H disaccharide | Homo sapiens | UDP + alpha-D-galactosyl-(1,3)-[alpha-L-fucosyl(1,2)]-D-galactose | - |
? | |
UDP-galactose + blood group antigen | GTB catalyzes the transfer of galactose from UDP-Gal to the C3 position of the terminal galactose of H antigen acceptors | Homo sapiens | UDP + alpha-D-galactosyl-blood group antigen | - |
? | |
UDP-galactose + blood group antigen H | GTB catalyzes the transfer of galactose from UDP-Gal to the C3 position of the terminal galactose of H antigen acceptors | Homo sapiens | UDP + alpha-D-galactosyl-blood group antigen H | - |
? |
Synonyms | Comment | Organism |
---|---|---|
blood group B galactosyltransferase | - |
Homo sapiens |
GTB | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | human blood group B galactosyltransferase responsible for the biosynthesis of human blood group antigens | Homo sapiens |