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Literature summary for 2.4.1.365 extracted from

  • Jung, S.; Kim, W.; Park, S.; Jeong, J.; Park, M.; Lim, S.; Lee, Y.; Im, W.; Lee, J.; Choi, G.; Kim, S.
    Two ginseng UDP-glycosyltransferases synthesize ginsenoside Rg 3 and Rd (2014), Plant Cell Physiol., 55, 2177-2188 .
    View publication on PubMed
Search for more literature for 2.4.1.365

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells and in Saccharomyces cerevisiae Panax ginseng

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.188
-
 ginsenoside F2 at pH 8.0 and 35°C Panax ginseng
0.311
-
 (20S)-ginsenoside Rh2 at pH 8.0 and 35°C Panax ginseng

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + (20S)-ginsenoside Rh2 Panax ginseng
-
 UDP + (20S)-ginsenoside Rg3
-
 ?
UDP-alpha-D-glucose + ginsenoside F2 Panax ginseng
-
 UDP + ginsenoside Rd
-
 ?

Organism

Organism UniProt Comment Textmining
Panax ginseng A0A0A6ZFY4
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione-Sepharose column chromatography Panax ginseng

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Panax ginseng
-
root highest expression Panax ginseng
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme glucosylates the C3-O-glucoside of both (20S)-ginsenoside Rh2 and ginsenoside F2, but not the C20-O-glucoside of ginsenoside C-K and ginsenoside F2, also displaying strong substrate specificity and regioselectivity Panax ginseng ?
-
 ?
UDP-alpha-D-glucose + (20S)-ginsenoside Rh2
-
 Panax ginseng UDP + (20S)-ginsenoside Rg3
-
 ?
UDP-alpha-D-glucose + ginsenoside F2
-
 Panax ginseng UDP + ginsenoside Rd
-
 ?

Synonyms

Synonyms Comment Organism
UGT94Q2
-
 Panax ginseng

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.41
-
 ginsenoside F2 at pH 8.0 and 35°C Panax ginseng
8.6
-
 (20S)-ginsenoside Rh2 at pH 8.0 and 35°C Panax ginseng

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.183
-
 ginsenoside F2 at pH 8.0 and 35°C Panax ginseng
27.683
-
 (20S)-ginsenoside Rh2 at pH 8.0 and 35°C Panax ginseng