Cloned (Comment) | Organism |
---|---|
the gene cloned into pQE80 L for expression | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.76 | - |
GDP-beta-L-colitose | pH 8.0, 37°C | Escherichia coli | |
1.3 | - |
GDP-L-fucose | pH 8.0, 37°C | Escherichia coli | |
1.4 | - |
beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine | pH 8.0, 37°C | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Escherichia coli | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme does not require a metal ion | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 30000, N-terminal Histagged, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-beta-L-colitose + beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine | Escherichia coli | beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine i.e. lacto-N-biose. The enzyme participates in the biosynthesis of an O-antigen | GDP + alpha-L-colitosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine | - |
? | |
GDP-beta-L-colitose + beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine | Escherichia coli O55:H5 | beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine i.e. lacto-N-biose. The enzyme participates in the biosynthesis of an O-antigen | GDP + alpha-L-colitosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli O55:H5 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-beta-L-colitose + beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine | beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine i.e. lacto-N-biose. The enzyme participates in the biosynthesis of an O-antigen | Escherichia coli | GDP + alpha-L-colitosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine | - |
? | |
GDP-beta-L-colitose + beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine | beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine i.e. lacto-N-biose. The enzyme is highly specific towards the acceptor, exclusively recognizing lacto-N-biose, but can accept GDP-L-fucose as the donor with almost the same activity as with GDP-beta-L-colitose | Escherichia coli | GDP + alpha-L-colitosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine | - |
? | |
GDP-beta-L-colitose + beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine | beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine i.e. lacto-N-biose. The enzyme participates in the biosynthesis of an O-antigen | Escherichia coli O55:H5 | GDP + alpha-L-colitosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine | - |
? | |
GDP-beta-L-colitose + beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine | beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine i.e. lacto-N-biose. The enzyme is highly specific towards the acceptor, exclusively recognizing lacto-N-biose, but can accept GDP-L-fucose as the donor with almost the same activity as with GDP-beta-L-colitose | Escherichia coli O55:H5 | GDP + alpha-L-colitosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine | - |
? | |
GDP-L-fucose + beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine | beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine i.e. lacto-N-biose. The enzyme is highly specific towards the acceptor, exclusively recognizing lacto-N-biose, but can accept GDP-L-fucose as the donor with almost the same activity as with GDP-beta-L-colitose | Escherichia coli | GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine | - |
? | |
GDP-L-fucose + beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine | beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine i.e. lacto-N-biose. The enzyme is highly specific towards the acceptor, exclusively recognizing lacto-N-biose, but can accept GDP-L-fucose as the donor with almost the same activity as with GDP-beta-L-colitose | Escherichia coli O55:H5 | GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, N-terminal Histagged, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
alpha1,2-ColT | - |
Escherichia coli |
wbgN | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.087 | - |
beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine | pH 8.0, 37°C | Escherichia coli | |
0.147 | - |
GDP-beta-L-colitose | pH 8.0, 37°C | Escherichia coli | |
0.2 | - |
GDP-L-fucose | pH 8.0, 37°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | - |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
10.2 | - |
about 60% of maximal activity | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.062 | - |
beta-D-galactopyranosyl-(1->3)-N-acetyl-D-glucosamine | pH 8.0, 37°C | Escherichia coli | |
0.15 | - |
GDP-L-fucose | pH 8.0, 37°C | Escherichia coli | |
0.193 | - |
GDP-beta-L-colitose | pH 8.0, 37°C | Escherichia coli |