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Literature summary for 2.4.1.260 extracted from

  • Massarweh, A.; Bosco, M.E.; Iatmanen-Harbi, S.; Tessier, C.; Amana, L.; Busca, P.; Chantret, I.; Gravier-Pelletier, C.; Moore, S.E.
    Brefeldin A promotes the appearance of oligosaccharyl phosphates (OSP) derived from Glc3Man9GlcNAc2-PP-dolichol within the endomembrane system of HepG2 cells (2016), J. Lipid Res., 57, 1477-1491.
    View publication on PubMedView publication on EuropePMC
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Organism

Organism UniProt Comment Textmining
Homo sapiens
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General Information

General Information Comment Organism
physiological function generation of a HepG2 cell model of Alg12-congenital disorders of glycosylation by down-regulating dolichyl-P-mannose Man7GlcNAc2-PP-dolichol mannosyltransferase (Alg12), in order to provoke the accumulation of Man7GlcNAc2-PP-dolichol. The accumulation of Man7GlcNAc2-PP-dolichol in ALG12-downregulated cells coincides with the inhibition in cell growth noted between 4 and 8 days posttransfection. In down-regulated cells, brefeldin A provokes Golgi apparatus-endomannosidase trimming of Glc3Man9GlcNAc2-PP dolichol to yield a Man8GlcNAc2-PP-dolichol structure that does not give rise to cytoplasmic Man8GlcNAc2-P. Brefeldin A also strikingly increases oligosaccharyl phosphates derived from mature diphosphodolichol within the endomembrane system without affecting levels of Man7GlcNAc2-PP-dolichol or cytoplasmic Man7GlcNAc2-P Homo sapiens