Cloned (Comment) | Organism |
---|---|
- |
Corynebacterium glutamicum |
Crystallization (Comment) | Organism |
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vapor diffusion under silicon oil. The structure of MshA is determined both in the absence of substrates and in a complex with UDP and 11D-myo-inositol 3-phosphate. The structure of unliganded CgMshA (APO form) is determined by single isomorphous replacement with anomalous scattering utilizing a CuKalpha home source and a mercury derivative to 2.1 A resolution. Crystallization of CgMshA in the presence of UDP or UDP-GlcNAc results in a tetragonal crystal form. The structure of MshA from Corynebacterium glutamicum is determined both in the absence of substrates and in a complex with UDP and 1L-myo-inositol-1-phosphate | Corynebacterium glutamicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
UDP-N-acetyl-D-glucosamine | pH 7.8, 25°C | Corynebacterium glutamicum | |
0.24 | - |
1D-myo-inositol 3-phosphate | pH 7.8, 25°C | Corynebacterium glutamicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate | Corynebacterium glutamicum | 1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate, committed step of mycothiol biosynthesis | 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Corynebacterium glutamicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate | 1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate, committed step of mycothiol biosynthesis | Corynebacterium glutamicum | 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP | - |
? | |
UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate | 1D-myo-inositol 3-phosphate i.e. 1L-myo-inositol 1-phosphate. Molecular models of the ternary complex suggest a mechanism in which the beta-phosphate of the substrate, UDP-N-acetylglucosamine, promotes the nucleophilic attack of the 3-hydroxyl group of 1D-myo-inositol 3-phosphate while at the same time promoting the cleavage of the sugar nucleotide bond | Corynebacterium glutamicum | 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CgMshA | - |
Corynebacterium glutamicum |