Application | Comment | Organism |
---|---|---|
nutrition | acting on gelatinized food-grade potato starch, PyAMase produced a thermoreversible starch product with gelatin-like properties. This thermoreversible gel has potential applications in the food industry | Pyrobaculum aerophilum |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrobaculum aerophilum |
General Stability | Organism |
---|---|
dithiothreitol decreases thermal stability of the enzyme | Pyrobaculum aerophilum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acarbose | - |
Pyrobaculum aerophilum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
maltoheptaose | pH 6.7, 80°C | Pyrobaculum aerophilum | |
2.1 | - |
maltotetraose | pH 6.7, 80°C | Pyrobaculum aerophilum | |
3.2 | - |
maltohexaose | pH 6.7, 80°C | Pyrobaculum aerophilum | |
3.7 | - |
maltotriose | pH 6.7, 80°C | Pyrobaculum aerophilum | |
6.9 | - |
maltopentaose | pH 6.7, 80°C | Pyrobaculum aerophilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrobaculum aerophilum | - |
IM2 | - |
Pyrobaculum aerophilum IM2 | - |
IM2 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Pyrobaculum aerophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltoheptaose + maltoheptaose | - |
Pyrobaculum aerophilum | D-glucose + maltooligosaccharides | - |
? | |
maltoheptaose + maltoheptaose | - |
Pyrobaculum aerophilum IM2 | D-glucose + maltooligosaccharides | - |
? | |
maltohexaose + maltohexaose | - |
Pyrobaculum aerophilum | D-glucose + maltooligosaccharides | - |
? | |
maltohexaose + maltohexaose | - |
Pyrobaculum aerophilum IM2 | D-glucose + maltooligosaccharides | - |
? | |
maltopentaose + maltopentaose | - |
Pyrobaculum aerophilum | D-glucose + maltooligosaccharides | - |
? | |
maltopentaose + maltopentaose | - |
Pyrobaculum aerophilum IM2 | D-glucose + maltooligosaccharides | - |
? | |
maltotetraose + maltotetraose | - |
Pyrobaculum aerophilum | D-glucose + maltooligosaccharides | - |
? | |
maltotetraose + maltotetraose | - |
Pyrobaculum aerophilum IM2 | D-glucose + maltooligosaccharides | - |
? | |
maltotriose + maltotriose | - |
Pyrobaculum aerophilum | D-glucose + maltopentaose | - |
? | |
additional information | acting on gelatinized food-grade potato starch, PyAMase produced a thermoreversible starch product with gelatin-like properties | Pyrobaculum aerophilum | ? | - |
? | |
additional information | acting on gelatinized food-grade potato starch, PyAMase produced a thermoreversible starch product with gelatin-like properties | Pyrobaculum aerophilum IM2 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
amylmaltase | - |
Pyrobaculum aerophilum |
PyAMase | - |
Pyrobaculum aerophilum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
- |
Pyrobaculum aerophilum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
87 | - |
half-life: 506 min | Pyrobaculum aerophilum |
89 | - |
half-life: 114 min | Pyrobaculum aerophilum |
95 | - |
half-life: 107 min | Pyrobaculum aerophilum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.7 | - |
maltoheptaose | pH 6.7, 80°C | Pyrobaculum aerophilum | |
8.9 | - |
maltohexaose | pH 6.7, 80°C | Pyrobaculum aerophilum | |
24.8 | - |
maltopentaose | pH 6.7, 80°C | Pyrobaculum aerophilum | |
51.2 | - |
maltotetraose | pH 6.7, 80°C | Pyrobaculum aerophilum | |
115 | - |
maltotriose | pH 6.7, 80°C | Pyrobaculum aerophilum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | - |
- |
Pyrobaculum aerophilum |