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Literature summary for 2.4.1.228 extracted from

  • Kaczmarek, R.; Mikolajewicz, K.; Szymczak, K.; Duk, M.; Majorczyk, E.; Krop-Watorek, A.; Buczkowska, A.; Czerwinski, M.
    Evaluation of an amino acid residue critical for the specificity and activity of human Gb3/CD77 synthase (2016), Glycoconj. J., 33, 963-973 .
    View publication on PubMedView publication on EuropePMC
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Protein Variants

Protein Variants Comment Organism
Q211E the mutation broadens the enzyme acceptor specificity, so the enzyme can not only attach galactose to another galactose but also to N-acetylgalactosamine. The latter reaction leads to synthesis of NOR antigens, which are glycosphingolipids with terminal Gal(alpha1-4)GalNAc sequence. Cells transfected with vectors encoding Gb3/CD77 synthase with substitutions p. Q211D or p. Q211N do not express Pk, P1 and NOR antigens, suggesting complete loss of enzymatic activity. Thus, amino acid residue at position 211 of Gb3/CD77 synthase is critical for specificity and activity of the enzyme involved in formation of Pk, P1 and NOR antigens Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9NPC4
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-

Synonyms

Synonyms Comment Organism
A4GalT
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 Homo sapiens
Gb3/CD77 synthase
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 Homo sapiens

General Information

General Information Comment Organism
metabolism the enzyme is responsible for biosynthesis of Gal(alpha1-4)Gal moiety in Gb3 (CD77, Pk antigen) and P1 glycosphingolipids Homo sapiens