Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.226 extracted from

  • Liao, W.C.; Liao, C.K.; Tseng, T.J.; Ho, Y.J.; Chen, Y.R.; Lin, K.H.; Lai, T.J.; Lan, C.T.; Wei, K.C.; Liu, C.H.
    Chondroitin sulfate synthase 1 enhances proliferation of glioblastoma by modulating PDGFRA stability (2020), Oncogenesis, 9, 9 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.226

Cloned(Commentary)

Cloned (Comment) Organism
gene CHSY1, quantitative RT-PCR enzyme expressiona analysis Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information both CHSY1 expression and CS56 staining are dramatically decreased in CHSY1-silenced cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Homo sapiens 5737
-
extracellular
-
 Homo sapiens
-
-
additional information CHSY1 is mainly expressed in the paranuclear cytoplasm and strong CS56 staining in the extracellular space, plasma membrane, and cytoplasm of control cells Homo sapiens
-
-
plasma membrane
-
 Homo sapiens 5886
-

Organism

Organism UniProt Comment Textmining
Homo sapiens Q86X52
-
-

Source Tissue

Source Tissue Comment Organism Textmining
A-172 cell
-
 Homo sapiens
-
astrocytoma cell
-
 Homo sapiens
-
GL-261 cell
-
 Homo sapiens
-
glioma cell CHSY1 is frequently upregulated in glioblastoma and correlates with high tumor grade and poor survival Homo sapiens
-
oligodendroglioma cell
-
 Homo sapiens
-
U-118MG cell
-
 Homo sapiens
-

Synonyms

Synonyms Comment Organism
bifunctional CS synthase
-
 Homo sapiens
chondroitin sulfate synthase 1
-
 Homo sapiens
CHSY1
-
 Homo sapiens
CS synthase 1
-
 Homo sapiens

Expression

Organism Comment Expression
Homo sapiens CHSY1 is frequently upregulated in human glioma up

General Information

General Information Comment Organism
malfunction correlation between clinicopathological features and expression of CS synthases in glioma patients. The changes of cell surface CS and its contribution to malignant growth of glioma cells are analyzed, by manipulating CS synthase 1 (CHSY1) expression. Overexpression of CHSY1 inhibits PDGF-triggered decrease in PDGFRA levels, whereas CHSY1 knockdown accelerates PDGFRA decrease following PDGF stimulation. PDGFRA is highly expressed on the cell membrane of CHSY1-overexpressing GL261 tumor tissue sections. PDGFRA inhibition reverses CHSY1-mediated tumor growth in vitro and in vivo Homo sapiens
metabolism the biosynthesis of chondroitin sulfate (CS) chains begins with the formation of a link between N-acetylgalactosamine (GalNAc) and a common tetrasaccharide structure at a serine residue on the core protein. The next step (polymerization) is catalyzed by a group of bifunctional enzymes that have beta1-3 glucuronosyltransferase and beta1-4 N-acetylgalactosaminyltransferase activities. A single CS chain can consist of up to 50 repeating GlcA-GalNAc subunits, which are modified with sulfate groups at various positions. Three bifunctional CS synthases, CHSY1, CHPF (CHSY2), and CHSY3, control polymerization of CS chains Homo sapiens
physiological function CHSY1 selectively modulates platelet derived growth factor receptor alpha (PDGFRA) signaling, and that survival of a mouse model of a CHSY1-expressing tumor is increased by using a PDGFR inhibitor. CHSY1 mediates CS formation in glioma cells. CHSY1 is a crucial enzyme to modulate CS formation in GBM cells in vitro. CHSY1 selectively regulates the PDGFRA pathway and enhances PDGFRA protein stability in glioblastoma cells Homo sapiens