Cloned (Comment) | Organism |
---|---|
gene CHSY1, quantitative RT-PCR enzyme expressiona analysis | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | both CHSY1 expression and CS56 staining are dramatically decreased in CHSY1-silenced cells | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
extracellular | - |
Homo sapiens | - |
- |
additional information | CHSY1 is mainly expressed in the paranuclear cytoplasm and strong CS56 staining in the extracellular space, plasma membrane, and cytoplasm of control cells | Homo sapiens | - |
- |
plasma membrane | - |
Homo sapiens | 5886 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q86X52 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
A-172 cell | - |
Homo sapiens | - |
astrocytoma cell | - |
Homo sapiens | - |
GL-261 cell | - |
Homo sapiens | - |
glioma cell | CHSY1 is frequently upregulated in glioblastoma and correlates with high tumor grade and poor survival | Homo sapiens | - |
oligodendroglioma cell | - |
Homo sapiens | - |
U-118MG cell | - |
Homo sapiens | - |
Synonyms | Comment | Organism |
---|---|---|
bifunctional CS synthase | - |
Homo sapiens |
chondroitin sulfate synthase 1 | - |
Homo sapiens |
CHSY1 | - |
Homo sapiens |
CS synthase 1 | - |
Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | CHSY1 is frequently upregulated in human glioma | up |
General Information | Comment | Organism |
---|---|---|
malfunction | correlation between clinicopathological features and expression of CS synthases in glioma patients. The changes of cell surface CS and its contribution to malignant growth of glioma cells are analyzed, by manipulating CS synthase 1 (CHSY1) expression. Overexpression of CHSY1 inhibits PDGF-triggered decrease in PDGFRA levels, whereas CHSY1 knockdown accelerates PDGFRA decrease following PDGF stimulation. PDGFRA is highly expressed on the cell membrane of CHSY1-overexpressing GL261 tumor tissue sections. PDGFRA inhibition reverses CHSY1-mediated tumor growth in vitro and in vivo | Homo sapiens |
metabolism | the biosynthesis of chondroitin sulfate (CS) chains begins with the formation of a link between N-acetylgalactosamine (GalNAc) and a common tetrasaccharide structure at a serine residue on the core protein. The next step (polymerization) is catalyzed by a group of bifunctional enzymes that have beta1-3 glucuronosyltransferase and beta1-4 N-acetylgalactosaminyltransferase activities. A single CS chain can consist of up to 50 repeating GlcA-GalNAc subunits, which are modified with sulfate groups at various positions. Three bifunctional CS synthases, CHSY1, CHPF (CHSY2), and CHSY3, control polymerization of CS chains | Homo sapiens |
physiological function | CHSY1 selectively modulates platelet derived growth factor receptor alpha (PDGFRA) signaling, and that survival of a mouse model of a CHSY1-expressing tumor is increased by using a PDGFR inhibitor. CHSY1 mediates CS formation in glioma cells. CHSY1 is a crucial enzyme to modulate CS formation in GBM cells in vitro. CHSY1 selectively regulates the PDGFRA pathway and enhances PDGFRA protein stability in glioblastoma cells | Homo sapiens |