Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.226 extracted from

  • Kitagawa, H.; Izumikawa, T.; Uyama, T.; Sugahara, K.
    Molecular cloning of a chondroitin polymerizing factor that cooperates with chondroitin synthase for chondroitin polymerization (2003), J. Biol. Chem., 278, 23666-23671.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
genetic organization, coexpression of enzyme and chondroitin polymerizing factor as fusion protein in COS-1 cells, secretion into the cell culture medium Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucuronate + chondroitin Homo sapiens chondroitin polymerization in disaccharide units by glucuronyltransferase and N-acetylgalactosaminyltransferase activities of the enzyme UDP + beta-D-glucuronosyl-(1,3)-chondroitin
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8IZ52
-
-

Reaction

Reaction Comment Organism Reaction ID
UDP-alpha-D-glucuronate + [protein]-3-O-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine = UDP + [protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine enzyme has dual function possessing both glucuronyltransferase and N-acetylgalactosaminyltransferase activities Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Homo sapiens
0.00000004
-
glucuronyltransferase of recombinant wild-type enzyme secreted from COS-1 cells Homo sapiens
0.00000314
-
glucuronyltransferase of recombinant mutant fusion protein consisting of enzyme and chondroitin polymerizing factor secreted from COS-1 cells Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information in vitro activity of the recombinant enzyme requires concomitant expression of the chondroitin polymerizing factor ChPF in a fusion protein, mixing of separately expressed proteins does not result in an active complex, overview Homo sapiens ?
-
?
UDP-alpha-D-glucuronate + alpha-thrombomodulin
-
Homo sapiens UDP + beta-D-glucuronosyl-(1,3)-alpha-thrombomodulin
-
?
UDP-alpha-D-glucuronate + beta-D-glucuronosyl-(1,3)-beta-D-galactosyl-(1,3)-beta-D-galactosyl-1,4-beta-D-xylose-1-O-Gly-Ser-Gly-Glu synthetic tetrasaccharide substrate Homo sapiens UDP + ?
-
?
UDP-alpha-D-glucuronate + beta-D-glucuronosyl-(1,3)-beta-D-galactosyl-(1,3)-beta-D-galactosyl-1,4-beta-D-xylose-1-O-serine synthetic tetrasaccharide substrate Homo sapiens UDP + ?
-
?
UDP-alpha-D-glucuronate + chondroitin
-
Homo sapiens UDP + beta-D-glucuronosyl-(1,3)-chondroitin
-
?
UDP-alpha-D-glucuronate + chondroitin chondroitin polymerization in disaccharide units by glucuronyltransferase and N-acetylgalactosaminyltransferase activities of the enzyme Homo sapiens UDP + beta-D-glucuronosyl-(1,3)-chondroitin
-
?

Synonyms

Synonyms Comment Organism
chondroitin synthase
-
Homo sapiens
ChSy
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
assay at Homo sapiens