Cloned (Comment) | Organism |
---|---|
genetic organization, coexpression of enzyme and chondroitin polymerizing factor as fusion protein in COS-1 cells, secretion into the cell culture medium | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucuronate + chondroitin | Homo sapiens | chondroitin polymerization in disaccharide units by glucuronyltransferase and N-acetylgalactosaminyltransferase activities of the enzyme | UDP + beta-D-glucuronosyl-(1,3)-chondroitin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q8IZ52 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-alpha-D-glucuronate + [protein]-3-O-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine = UDP + [protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine | enzyme has dual function possessing both glucuronyltransferase and N-acetylgalactosaminyltransferase activities | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
0.00000004 | - |
glucuronyltransferase of recombinant wild-type enzyme secreted from COS-1 cells | Homo sapiens |
0.00000314 | - |
glucuronyltransferase of recombinant mutant fusion protein consisting of enzyme and chondroitin polymerizing factor secreted from COS-1 cells | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | in vitro activity of the recombinant enzyme requires concomitant expression of the chondroitin polymerizing factor ChPF in a fusion protein, mixing of separately expressed proteins does not result in an active complex, overview | Homo sapiens | ? | - |
? | |
UDP-alpha-D-glucuronate + alpha-thrombomodulin | - |
Homo sapiens | UDP + beta-D-glucuronosyl-(1,3)-alpha-thrombomodulin | - |
? | |
UDP-alpha-D-glucuronate + beta-D-glucuronosyl-(1,3)-beta-D-galactosyl-(1,3)-beta-D-galactosyl-1,4-beta-D-xylose-1-O-Gly-Ser-Gly-Glu | synthetic tetrasaccharide substrate | Homo sapiens | UDP + ? | - |
? | |
UDP-alpha-D-glucuronate + beta-D-glucuronosyl-(1,3)-beta-D-galactosyl-(1,3)-beta-D-galactosyl-1,4-beta-D-xylose-1-O-serine | synthetic tetrasaccharide substrate | Homo sapiens | UDP + ? | - |
? | |
UDP-alpha-D-glucuronate + chondroitin | - |
Homo sapiens | UDP + beta-D-glucuronosyl-(1,3)-chondroitin | - |
? | |
UDP-alpha-D-glucuronate + chondroitin | chondroitin polymerization in disaccharide units by glucuronyltransferase and N-acetylgalactosaminyltransferase activities of the enzyme | Homo sapiens | UDP + beta-D-glucuronosyl-(1,3)-chondroitin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
chondroitin synthase | - |
Homo sapiens |
ChSy | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Homo sapiens |