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Literature summary for 2.4.1.212 extracted from
- Characterization of the purified hyaluronan synthase from Streptococcus equisimilis (2004), Biochemistry, 43, 9234-9242.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | enzyme is more active in 25 mM phosphate buffer than in 50 mM | Streptococcus dysgalactiae subsp. equisimilis | |
| tetraoleoyl cardiolipin | highly activating | Streptococcus dysgalactiae subsp. equisimilis |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Streptococcus dysgalactiae subsp. equisimilis |
General Stability
| General Stability | Organism |
|---|---|
| substrate and cardiolipin stabilize the enzyme | Streptococcus dysgalactiae subsp. equisimilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ethylene glycol | - |
Streptococcus dysgalactiae subsp. equisimilis | |
| glycerol | - |
Streptococcus dysgalactiae subsp. equisimilis | |
| additional information | enzyme is more active in 25 mM phosphate buffer than in 50 mM, enzyme is inhibited by increasing viscosity via addition of PEG, glycerol, sucrose or ethylene glycol | Streptococcus dysgalactiae subsp. equisimilis | |
| PEG | increasing MW increases the inhibitory effect on the enzyme, overview | Streptococcus dysgalactiae subsp. equisimilis | |
| PEG 20000 | - |
Streptococcus dysgalactiae subsp. equisimilis | |
| sucrose | - |
Streptococcus dysgalactiae subsp. equisimilis | |
| tetramyristoyl cardiolipin | inactivating | Streptococcus dysgalactiae subsp. equisimilis | |
| UDP | - |
Streptococcus dysgalactiae subsp. equisimilis | |
| UDP-Gal | - |
Streptococcus dysgalactiae subsp. equisimilis | |
| UDP-GalNAc | - |
Streptococcus dysgalactiae subsp. equisimilis | |
| UDP-GalUA | - |
Streptococcus dysgalactiae subsp. equisimilis | |
| UDP-Glc | - |
Streptococcus dysgalactiae subsp. equisimilis | |
| UMP | - |
Streptococcus dysgalactiae subsp. equisimilis | |
| UTP | - |
Streptococcus dysgalactiae subsp. equisimilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | recombinant enzyme, kinetics at different pH, thermodynamics | Streptococcus dysgalactiae subsp. equisimilis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Streptococcus dysgalactiae subsp. equisimilis | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | activates at 50 mM, slightly more effective than NaCl | Streptococcus dysgalactiae subsp. equisimilis | |
| Mg2+ | required | Streptococcus dysgalactiae subsp. equisimilis | |
| NaCl | activates at 50 mM, slightly less effective than KCl | Streptococcus dysgalactiae subsp. equisimilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49000 | - |
x * 49000, recombinant enzyme, SDS-PAGE | Streptococcus dysgalactiae subsp. equisimilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-D-glucosamine + UDP-D-glucuronate | Streptococcus dysgalactiae subsp. equisimilis | - |
[beta-N-acetyl-D-glucosaminyl(1-4)beta-D-glucuronosyl(1-3)]n + UDP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus dysgalactiae subsp. equisimilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from membranes of Escherichia coli, to homogeneity | Streptococcus dysgalactiae subsp. equisimilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme is inactive without bound cardiolipins | Streptococcus dysgalactiae subsp. equisimilis | ? | - |
? | |
| UDP-N-acetyl-D-glucosamine + UDP-D-glucuronate | - |
Streptococcus dysgalactiae subsp. equisimilis | [beta-N-acetyl-D-glucosaminyl(1-4)beta-D-glucuronosyl(1-3)]n + UDP | - |
? | |
| UDP-N-acetyl-D-glucosamine + UDP-D-glucuronate | highly specific for UDP-N-acetyl-D-glucosamine and UDP-D-glucuronate, addition of monosaccharides to the heteropolysaccharide chain consisting of repeated disaccharides up to 25000 | Streptococcus dysgalactiae subsp. equisimilis | [beta-N-acetyl-D-glucosaminyl(1-4)beta-D-glucuronosyl(1-3)]n + UDP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 49000, recombinant enzyme, SDS-PAGE | Streptococcus dysgalactiae subsp. equisimilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HAS | - |
Streptococcus dysgalactiae subsp. equisimilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Streptococcus dysgalactiae subsp. equisimilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the enzyme is temperature labile, but is stabilized by substrate and cardiolipin | Streptococcus dysgalactiae subsp. equisimilis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 120 | - |
UDP-D-glucuronate | recombinant enzyme, pH 7.0, 30°C | Streptococcus dysgalactiae subsp. equisimilis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 10 | recombinant enzyme, pH-profile | Streptococcus dysgalactiae subsp. equisimilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 11.5 | recombinant enzyme, pH-profile | Streptococcus dysgalactiae subsp. equisimilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
1 h, at 30°C, loss of 74-90% activity | Streptococcus dysgalactiae subsp. equisimilis |
| 11.5 | - |
1 h, at 30°C, loss of 40-50% activity | Streptococcus dysgalactiae subsp. equisimilis |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
sucrose | recombinant enzyme, pH 7.0, 30°C | Streptococcus dysgalactiae subsp. equisimilis | |
| 3.3 | - |
glycerol | recombinant enzyme, pH 7.0, 30°C | Streptococcus dysgalactiae subsp. equisimilis | |
| 3.5 | - |
PEG 20000 | recombinant enzyme, pH 7.0, 30°C | Streptococcus dysgalactiae subsp. equisimilis | |
| 4.5 | - |
ethylene glycol | recombinant enzyme, pH 7.0, 30°C | Streptococcus dysgalactiae subsp. equisimilis | |
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