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Literature summary for 2.4.1.21 extracted from

  • Nielsen, M.M.; Ruzanski, C.; Krucewicz, K.; Striebeck, A.; Cenci, U.; Ball, S.G.; Palcic, M.M.; Cuesta-Seijo, J.A.
    Crystal structures of the catalytic domain of Arabidopsis thaliana starch synthase IV, of granule bound starch synthase from CLg1 and of granule bound starch synthase I of Cyanophora paradoxa illustrate substrate recognition in starch synthases (2018), Front. Plant Sci., 9, 1138 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.21

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Tuner(DE3) cells Cyanobacterium sp. CLg1
expressed in Escherichia coli Tuner(DE3) cells Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment) Organism
catalytic domain bound to ADP and inhibitor acarbose, hanging drop vapor diffusion method, using 0.2 MLi2SO4, 0.1MBis-Tris pH 5.5, 25% (w/v) PEG3350 Arabidopsis thaliana
catalytic domain bound to ADP and inhibitor acarbose, hanging drop vapor diffusion method, using 2 M (NH4)2SO4, 2% (w/v) PEG400 and 150 mM HEPES pH 7.5 Cyanobacterium sp. CLg1

Inhibitors

Inhibitors Comment Organism Structure
acarbose
-
 Arabidopsis thaliana
acarbose
-
 Cyanobacterium sp. CLg1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP-alpha-D-glucose + [(1->4)-alpha-D-glucosyl]n Cyanobacterium sp. CLg1
-
 ADP + [(1->4)-alpha-D-glucosyl]n+1
-
 ?
ADP-alpha-D-glucose + [(1->4)-alpha-D-glucosyl]n Arabidopsis thaliana
-
 ADP + [(1->4)-alpha-D-glucosyl]n+1
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q0WVX5
-
-
Cyanobacterium sp. CLg1 V5SNJ5
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex 75 gel filtration Cyanobacterium sp. CLg1
HisTrap column chromatography and Superdex 75 gel filtration Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-alpha-D-glucose + [(1->4)-alpha-D-glucosyl]n
-
 Cyanobacterium sp. CLg1 ADP + [(1->4)-alpha-D-glucosyl]n+1
-
 ?
ADP-alpha-D-glucose + [(1->4)-alpha-D-glucosyl]n
-
 Arabidopsis thaliana ADP + [(1->4)-alpha-D-glucosyl]n+1
-
 ?

Synonyms

Synonyms Comment Organism
GBSS
-
 Cyanobacterium sp. CLg1
granule bound starch synthase
-
 Cyanobacterium sp. CLg1
starch synthase IV
-
 Arabidopsis thaliana