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Literature summary for 2.4.1.207 extracted from

  • Li, Q.; Li, H.; Yin, C.; Wang, X.; Jiang, Q.; Zhang, R.; Ge, F.; Chen, Y.; Yang, L.
    Genome-wide identification and characterization of xyloglucan endotransglycosylase/hydrolase in ananas comosus during development (2019), Genes (Basel), 10, 537 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
identification of several genes designated Ac(F153)XTH1 to Ac(F153)XTH24, sequence comparisons and phylogenetic analysis, genetic structure analysis and pattern of the motif in XTHs, genome-wide analysis, quantitative real-time PCR expression analysis. Detailed overview Ananas comosus
identification of several genes designated Ac(MD2)XTH1 to Ac(MD2)XTH24, sequence comparisons and phylogenetic analysis, genetic structure analysis and pattern of the motif in XTHs, genome-wide analysis, quantitative real-time PCR expression analysis. Detailed overview Ananas comosus

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall XTH1-24 Ananas comosus 5618
-
cytoplasm XTH2, 3, 4, 5, 9, 10, and 13 Ananas comosus 5737
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cytoplasm XTH2, 3, and 9-13 Ananas comosus 5737
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Organism

Organism UniProt Comment Textmining
Ananas comosus
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cultivar F153
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Ananas comosus
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cultivar MD2
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
Ananas comosus
-
leaf
-
Ananas comosus
-
leaf highest expression of Ac(MD2)2XTH13 Ananas comosus
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additional information Ac(MD2)XTH15 shows the highest relative expression level in the green leaf tip, sequentially in the root, and finally in the white leaf base Ananas comosus
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root highest expression of Ac(MD2)XTH18 Ananas comosus
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Subunits

Subunits Comment Organism
? x * 25990-64580, XTH1-24, sequence calculation Ananas comosus
? x * 30560-39410, XTH1-24, sequence calculation Ananas comosus

Synonyms

Synonyms Comment Organism
Ac(F153)XTH1
-
Ananas comosus
Ac(MD2)XTH1
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Ananas comosus
XTH1
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Ananas comosus

pI Value

Organism Comment pI Value Maximum pI Value
Ananas comosus XTH1-24, sequence calculation 9.04 4.68
Ananas comosus XTH1-24, sequence calculation 9.53 4.69

General Information

General Information Comment Organism
evolution endoglucanase family 16 members are identified in addition to XTHs of glycoside hydrolase family 16. Phylogenetic analysis clusters the XTHs into three major groups (group I/II, III and ancestral group), group III is subdivided into group IIIA and group IIIB. Similar gene structure and motif number are observed within a group. Two highly conserved domains, glycosyl hydrolase family 16 (GH16-XET) and xyloglucan endotransglycosylase C-terminus (C-XET), are detected by multiple sequences alignment of all XTHs. Segmental replication are detected in the two cultivars, with only the paralogous pair Ac(F153)XTH7-Ac(F153)XTH18 presented in F153 prior to genomic expansion. Genome-wide analysis. Ka/Ks analysis and estimated divergence time of XTHs, detailed phylogenetic analysis and evolutionary relationships, overview Ananas comosus
evolution endoglucanase family 16 members are identified in addition to XTHs of glycoside hydrolase family 16. Phylogenetic analysis clusters the XTHs into three major groups (group I/II, III and ancestral group), group III is subdivided into group IIIA and group IIIB. Similar gene structure and motif number are observed within a group. Two highly conserved domains, glycosyl hydrolase family 16 (GH16-XET) and xyloglucan endotransglycosylase C-terminus (C-XET), are detected by multiple sequences alignment of all XTHs. Segmental replication are detected in the two cultivars, with only the paralogous pair Ac(F153)XTH7-Ac(F153)XTH18 presented in F153 prior to genomic expansion. Ka/Ks analysis and estimated divergence time of XTHs, detailed phylogenetic analysis and evolutionary relationships, overview Ananas comosus
physiological function transcriptomic analysis indicates that XTHs are involved in the regulation of fruit ripening and crassulacean acid metabolism with tissue specificity, and quantitative real-time PCR expression analysis suggests that Ac(MD2)XTH18 is involved in root growth. XTH proteins perform two diverse catalytic activities: xyloglucan endohydrolase (XEH) and xyloglucan endotransglycosylase (XET) Ananas comosus
physiological function transcriptomic analysis indicates that XTHs are involved in the regulation of fruit ripening and crassulacean acid metabolism with tissue specificity. XTH proteins perform two diverse catalytic activities: xyloglucan endohydrolase (XEH) and xyloglucan endotransglycosylase (XET) Ananas comosus