Cloned (Comment) | Organism |
---|---|
AtXTH12, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris strain GS115 | Arabidopsis thaliana |
AtXTH13, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris strain GS115. AtXTH13 cDNA construct is correctly expressed and posttranslationally modified with variable N-linked glycans on one site | Arabidopsis thaliana |
AtXTH17, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris strain GS115 | Arabidopsis thaliana |
AtXTH18, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris strain GS115 | Arabidopsis thaliana |
AtXTH19, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris strain GS115 | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics for AtXTH12, overviews | Arabidopsis thaliana | |
additional information | - |
additional information | Michaelis-Menten kinetics for AtXTH13, overviews | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell wall | - |
Arabidopsis thaliana | 5618 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Arabidopsis thaliana | root-specific isozyme AtXTH1 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan | ? | - |
? | |
additional information | Arabidopsis thaliana | root-specific isozyme AtXTH13 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. The endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan | ? | - |
? | |
additional information | Arabidopsis thaliana | root-specific isozyme AtXTH17 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan | ? | - |
? | |
additional information | Arabidopsis thaliana | root-specific isozyme AtXTH18 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan | ? | - |
? | |
additional information | Arabidopsis thaliana | root-specific isozyme AtXTH19 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O80803 | isozyme AtXTH17; 33 XTH genes | - |
Arabidopsis thaliana | Q9FKL8 | isozyme AtXTH13; 33 XTH genes | - |
Arabidopsis thaliana | Q9FKL9 | isozyme AtXTH12; 33 XTH genes | - |
Arabidopsis thaliana | Q9M0D1 | isozyme AtXTH19; 33 XTH genes | - |
Arabidopsis thaliana | Q9M0D2 | isozyme AtXTH18; 33 XTH genes | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | AtXTH13 cDNA construct is correctly expressed and posttranslationally modified with variable N-linked glycans on one site | Arabidopsis thaliana |
Purification (Comment) | Organism |
---|---|
recombinant AtXTH12 partially from Pichia pastoris by ammonium sulfate fractionation | Arabidopsis thaliana |
recombinant AtXTH13 partially from Pichia pastoris by ammonium sulfate fractionation, AtXTH13 is purified to apparent homogeneity by xylogluco-oligosaccharide affinity chromatography | Arabidopsis thaliana |
recombinant AtXTH17 partially from Pichia pastoris by ammonium sulfate fractionation | Arabidopsis thaliana |
recombinant AtXTH18 partially from Pichia pastoris by ammonium sulfate fractionation | Arabidopsis thaliana |
recombinant AtXTH19 partially from Pichia pastoris by ammonium sulfate fractionation | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | five predominantly root-expressed Arabidopsis thaliana XTHs belong to subgroup I/II, AtXTH12 is trichoblast-enriched | Arabidopsis thaliana | - |
root | five predominantly root-expressed Arabidopsis thaliana XTHs belong to subgroup I/II, AtXTH13 is trichoblast-enriched | Arabidopsis thaliana | - |
root | five predominantly root-expressed Arabidopsis thaliana XTHs belong to subgroup I/II, AtXTH17 is expressed in all cell types in the elongating and differentiating regions of the root | Arabidopsis thaliana | - |
root | five predominantly root-expressed Arabidopsis thaliana XTHs belong to subgroup I/II, AtXTH18 is expressed in all cell types in the elongating and differentiating regions of the root | Arabidopsis thaliana | - |
root | five predominantly root-expressed Arabidopsis thaliana XTHs belong to subgroup I/II, AtXTH19 is expressed in nearly all cell types in the root | Arabidopsis thaliana | - |
root hair | comparisons of mRNA populations of Arabidopsis root hair cells and cells of an Arabidopsis root hair mutant show AtXTH12 to be 4fold upregulated in root hair cells | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | root-specific isozyme AtXTH1 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan | Arabidopsis thaliana | ? | - |
? | |
additional information | root-specific isozyme AtXTH13 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. The endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan | Arabidopsis thaliana | ? | - |
? | |
additional information | root-specific isozyme AtXTH17 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan | Arabidopsis thaliana | ? | - |
? | |
additional information | root-specific isozyme AtXTH18 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan | Arabidopsis thaliana | ? | - |
? | |
additional information | root-specific isozyme AtXTH19 exhibits only the endotransglucosylase activity, XET, EC 2.4.1.207, towards xyloglucan and non-detectable endohydrolytic activity, XEH, EC 3.2.1.151. Its endotransglucosylase activity is preferentially directed towards xyloglucan and water-soluble cellulose acetate, rather than to mixed-linkage beta-glucan | Arabidopsis thaliana | ? | - |
? | |
additional information | XTH isozyme spcificities with different donor substrates, tamarind xyloglucan-derived oligosacchrides, overview | Arabidopsis thaliana | ? | - |
? | |
additional information | XTH isozyme spcificities with different donor substrates, tamarind xyloglucan-derived oligosacchrides, overview. AtXTH13 catalyzes the release of XGO1 (Glc4-based xylogluco-oligosaccharides) and XGO3 (Glc12-based xyloglucooligosaccharides) from a mixture of minimal XET/XEH substrates, XGO2 (Glc8-based xylogluco-oligosaccharides), derived from tamarind seed xyloglucan and bearing naturally variable galactosylation. The rate of release of XGO1 is identical to that of XGO3 at all substrate concentrations across a wide range | Arabidopsis thaliana | ? | - |
? | |
additional information | XTH isozyme specificity with different donor substrates, tamarind xyloglucan-derived oligosaccharides, overview | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AtXTH12 | - |
Arabidopsis thaliana |
AtXTH13 | - |
Arabidopsis thaliana |
AtXTH17 | - |
Arabidopsis thaliana |
AtXTH18 | - |
Arabidopsis thaliana |
AtXTH19 | - |
Arabidopsis thaliana |
XTH | - |
Arabidopsis thaliana |
xyloglucan endotransglucosylase/hydrolase | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 25 | - |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
AtXTH12 | Arabidopsis thaliana |
6 | - |
AtXTH13 | Arabidopsis thaliana |
7 | - |
AtXTH19 | Arabidopsis thaliana |
7.2 | - |
AtXTH17 | Arabidopsis thaliana |
7.5 | - |
AtXTH18 | Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | AtXTH13 is more tolerant to basic environments, retaining 40% of its maximal activity at pH 8.0, but it displays only 20% activity at pH 4.0. The enzyme is inactive at pH 3.5. Bell-shaped curve of AtXTH13 | Arabidopsis thaliana |
4 | 8 | bell-shaped curve of AtXTH12 | Arabidopsis thaliana |
5.5 | 8 | the activity-pH profile of AtXTH17 totally shifts towards the alkali side with its optimum at pH 7.2 and with an unusual acid intolerance. AtXTH18 shows the highest acid tolerance of these three enzymes. At pH 6.0 the enzyme still displays 60% of its activity at pH 7.0, whereas the activities of AtXTH17 and AtXTH19 drop below 30% of their maximal activity at pH 6.0. At or below pH 5.5, all three enzymes display less than 20% of their maximal activities. On the basic side, AtXTH17 shows the highest tolerance with activity of more than 80% of maximal activity at pH 8.0 | Arabidopsis thaliana |
5.5 | 8 | the activity-pH profile of AtXTH18 totally shifts towards the alkali side with optimum at pH 7.5 and with an unusual acid intolerance. At pH 6.0 the enzyme still displays 60% of its activity at pH 7.0. At or below pH 5.5, the enzyme displays less than 20% of its maximal activity. On the basic side, AtXTH18 shows the highest tolerance with activities of more than 80% of its maximal activity at pH 8.0 | Arabidopsis thaliana |
5.5 | 8 | the activity-pH profile of AtXTH19 totally shifts towards the alkali side with optimum at pH 7.0 and with an unusual acid intolerance. Activity of AtXTH19 drops below 30% of its maximal activity at pH 6.0. At or below pH 5.5, the enzymes displays less than 20% of its maximal activities. AtXTH19 retains 90% of its maximal activity at pH 7.5, but shows a 40% activity drop between pH 7.5 and pH 8.0 | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
physiological function | xyloglucan endotransglucosylase/hydrolases are cell wall enzymes that are able to graft xyloglucan chains to oligosaccharides or to other available xyloglucan chains and/or to hydrolyse xyloglucan chains. As they are involved in the modification of the load-bearing cell-wall components, they are believed to be very important in the regulation of growth and development | Arabidopsis thaliana |
physiological function | xyloglucan endotransglucosylase/hydrolases are cell wall enzymes that are able to graft xyloglucan chains to oligosaccharides or to other available xyloglucan chains and/or to hydrolyse xyloglucan chains. As they are involved in the modification of the load-bearing cell-wall components, they are believed to be very important in the regulation of growth and development. AtXTH13 might play a role later in root hair development | Arabidopsis thaliana |
physiological function | xyloglucan endotransglucosylase/hydrolases are cell wall enzymes that are able to graft xyloglucan chains to oligosaccharides or to other available xyloglucan chains and/or to hydrolyse xyloglucan chains. As they are involved in the modification of the load-bearing cell-wall components, they are believed to be very important in the regulation of growth and development. Role for AtXTH12 in root hair initiation. AtXTH13 might play a role later in root hair development | Arabidopsis thaliana |