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Literature summary for 2.4.1.183 extracted from

  • Tokashiki, J.; Hayashi, R.; Yano, S.; Watanabe, T.; Yamada, O.; Toyama, H.; Mizutani, O.
    Influence of alpha-1,3-glucan synthase gene agsE on protoplast formation for transformation of Aspergillus luchuensis (2019), J. Biosci. Bioeng., 128, 129-134 .
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information disruption of gene agsE in Aspergillus luchuensis (DELTAagsE) with Agrobacterium-mediated transformation (AMT) . The DELTAagsE protoplasts are also competent for transformation with the protoplast-PEG method. Phenotypes of the agsE disruptant, overview Aspergillus luchuensis

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall
-
Aspergillus luchuensis 5618
-
protoplast
-
Aspergillus luchuensis
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + [alpha-D-glucosyl-(1->3)]n Aspergillus luchuensis
-
UDP + [alpha-D-glucosyl-(1->3)]n+1
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus luchuensis A0A146F7N5
-
-

Purification (Commentary)

Purification (Comment) Organism
protoplast generation in Aspergillus luchuensis wild-type and DELTAagsE strains Aspergillus luchuensis

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Aspergillus luchuensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + [alpha-D-glucosyl-(1->3)]n
-
Aspergillus luchuensis UDP + [alpha-D-glucosyl-(1->3)]n+1
-
?

Synonyms

Synonyms Comment Organism
agsE
-
Aspergillus luchuensis

General Information

General Information Comment Organism
evolution AgsE is one of five alpha-1,3-glucan synthase genes in Aspergillus luchuensis and a homologue of the major alpha-1,3-glucan synthase agsB in Aspergillus nidulans Aspergillus luchuensis
malfunction disruption of agsE in Aspergillus luchuensis strain NBRC 4314 (DELTAagsE) shows that protoplast formation in DELTAagsE is comparable with protoplast formation in Aspergillus oryzae with commercial cell wall-digesting enzyme Yatalase. The DELTAagsE protoplasts are also competent for transformation with the protoplast-PEG method Aspergillus luchuensis
physiological function role of the alpha-1,3-glucan synthase gene agsE in protoplast formation: agsE appears to inhibit protoplast formation in Aspergillus luchuensis, overview Aspergillus luchuensis