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Literature summary for 2.4.1.18 extracted from
- Met349 mutations enhance the activity of 1,4-alpha-glucan branching enzyme from Geobacillus thermoglucosidans STB02 (2017), J. Agric. Food Chem., 65, 5674-5680 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Parageobacillus thermoglucosidasius |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| M349H | the mutant shows a slight decrease in specific activity compared with that of wild type enzyme | Parageobacillus thermoglucosidasius |
| M349S | the mutant shows 21.1% increase in specific activity compared with that of wild type enzyme. The mutant displays 24.2% enhancement in the alpha-1,6-glycosidic linkage ratio of potato starch samples | Parageobacillus thermoglucosidasius |
| M349T | the mutant shows 24.5% increase in specific activity compared with that of wild type enzyme. The mutant displays 24.2% enhancement in the alpha-1,6-glycosidic linkage ratio of potato starch samples | Parageobacillus thermoglucosidasius |
| M349Y | the mutant displays a significant (33.9%) reduction in specific activity compared with that of wild type enzyme | Parageobacillus thermoglucosidasius |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Parageobacillus thermoglucosidasius | A0A068F9H3 | - |
- |
| Parageobacillus thermoglucosidasius STB02 | A0A068F9H3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HiTrap chelating column chromatography | Parageobacillus thermoglucosidasius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| potato amylopectin type III | - |
Parageobacillus thermoglucosidasius | potato amylopectin type III containing alpha-1,6-glucosidic linkages | - |
? |  |
| potato amylopectin type III | - |
Parageobacillus thermoglucosidasius STB02 | potato amylopectin type III containing alpha-1,6-glucosidic linkages | - |
? | |
| potato amylose type III | - |
Parageobacillus thermoglucosidasius | potato amylose type III containing alpha-1,6-glucosidic linkages | - |
? | |
| potato amylose type III | - |
Parageobacillus thermoglucosidasius STB02 | potato amylose type III containing alpha-1,6-glucosidic linkages | - |
? | |
| potato starch | - |
Parageobacillus thermoglucosidasius | potato starch containing alpha-1,6-glucosidic linkages | - |
? | |
| potato starch | - |
Parageobacillus thermoglucosidasius STB02 | potato starch containing alpha-1,6-glucosidic linkages | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GBE | - |
Parageobacillus thermoglucosidasius |
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Release 2023.1 (January 2023)
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