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Literature summary for 2.4.1.18 extracted from

  • Liu, Y.; Li, C.; Gu, Z.; Xin, C.; Cheng, L.; Hong, Y.; Li, Z.
    Alanine 310 is important for the activity of 1,4-alpha-glucan branching enzyme from Geobacillus thermoglucosidans STB02 (2017), Int. J. Biol. Macromol., 97, 156-163 .
    View publication on PubMed
Search for more literature for 2.4.1.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Parageobacillus thermoglucosidasius

Protein Variants

Protein Variants Comment Organism
A310D the mutant shows strongly reduced activity compared to the wild type enzyme Parageobacillus thermoglucosidasius
A310E the mutant shows strongly reduced activity compared to the wild type enzyme Parageobacillus thermoglucosidasius
A310G the mutant shows strongly reduced activity compared to the wild type enzyme Parageobacillus thermoglucosidasius
A310I the mutant shows strongly reduced activity compared to the wild type enzyme Parageobacillus thermoglucosidasius
A310N the mutant shows strongly reduced activity compared to the wild type enzyme Parageobacillus thermoglucosidasius
A310Q the mutant shows strongly reduced activity compared to the wild type enzyme Parageobacillus thermoglucosidasius

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycogen Parageobacillus thermoglucosidasius
-
 glycogen containing alpha-1,6-glucosidic linkages
-
 ?
glycogen Parageobacillus thermoglucosidasius STB02
-
 glycogen containing alpha-1,6-glucosidic linkages
-
 ?
starch Parageobacillus thermoglucosidasius
-
 starch containing alpha-1,6-glucosidic linkages
-
 ?
starch Parageobacillus thermoglucosidasius STB02
-
 starch containing alpha-1,6-glucosidic linkages
-
 ?

Organism

Organism UniProt Comment Textmining
Parageobacillus thermoglucosidasius A0A068F9H3
-
-
Parageobacillus thermoglucosidasius STB02 A0A068F9H3
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap column chromatography and Superdex S200 gel filtration Parageobacillus thermoglucosidasius

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
26
-
 mutant enzyme A310G, using potato amylopectin type III as substrate, at pH 7.5 and 37°C Parageobacillus thermoglucosidasius
27
-
 mutant enzyme A310N, using potato amylopectin type III as substrate, at pH 7.5 and 37°C Parageobacillus thermoglucosidasius
27
-
 mutant enzyme A310Q, using potato amylopectin type III as substrate, at pH 7.5 and 37°C Parageobacillus thermoglucosidasius
30
-
 mutant enzyme A310I, using potato amylopectin type III as substrate, at pH 7.5 and 37°C Parageobacillus thermoglucosidasius
32
-
 mutant enzyme A310D, using potato amylopectin type III as substrate, at pH 7.5 and 37°C Parageobacillus thermoglucosidasius
32
-
 mutant enzyme A310E, using potato amylopectin type III as substrate, at pH 7.5 and 37°C Parageobacillus thermoglucosidasius
54
-
 mutant enzyme A310N, using potato amylose type III as substrate, at pH 7.5 and 37°C Parageobacillus thermoglucosidasius
58
-
 mutant enzyme A310G, using potato amylose type III as substrate, at pH 7.5 and 37°C Parageobacillus thermoglucosidasius
62
-
 mutant enzyme A310I, using potato amylose type III as substrate, at pH 7.5 and 37°C Parageobacillus thermoglucosidasius
62
-
 mutant enzyme A310Q, using potato amylose type III as substrate, at pH 7.5 and 37°C Parageobacillus thermoglucosidasius
67
-
 mutant enzyme A310E, using potato amylose type III as substrate, at pH 7.5 and 37°C Parageobacillus thermoglucosidasius
70
-
 mutant enzyme A310D, using potato amylose type III as substrate, at pH 7.5 and 37°C Parageobacillus thermoglucosidasius
299
-
 wild type enzyme, using potato amylose type III as substrate, at pH 7.5 and 37°C Parageobacillus thermoglucosidasius
384
-
 wild type enzyme, using potato amylopectin type III as substrate, at pH 7.5 and 37°C Parageobacillus thermoglucosidasius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycogen
-
 Parageobacillus thermoglucosidasius glycogen containing alpha-1,6-glucosidic linkages
-
 ?
glycogen
-
 Parageobacillus thermoglucosidasius STB02 glycogen containing alpha-1,6-glucosidic linkages
-
 ?
potato amylopectin type III
-
 Parageobacillus thermoglucosidasius potato amylopectin type III containing alpha-1,6-glucosidic linkages
-
 ?
potato amylopectin type III
-
 Parageobacillus thermoglucosidasius STB02 potato amylopectin type III containing alpha-1,6-glucosidic linkages
-
 ?
potato amylose type III
-
 Parageobacillus thermoglucosidasius potato amylose type III containing alpha-1,6-glucosidic linkages
-
 ?
potato amylose type III
-
 Parageobacillus thermoglucosidasius STB02 potato amylose type III containing alpha-1,6-glucosidic linkages
-
 ?
starch
-
 Parageobacillus thermoglucosidasius starch containing alpha-1,6-glucosidic linkages
-
 ?
starch
-
 Parageobacillus thermoglucosidasius STB02 starch containing alpha-1,6-glucosidic linkages
-
 ?

Subunits

Subunits Comment Organism
? x * 75000, SDS-PAGE Parageobacillus thermoglucosidasius

Synonyms

Synonyms Comment Organism
GBE
-
 Parageobacillus thermoglucosidasius