Cloned (Comment) | Organism |
---|---|
gene GmUGT1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, genotyping, quantitative real-time RT-PCR enzyme expression analysis | Glycine max |
gene GmUGT3, DNA and amino acid sequence determination and analysis, phylogenetic analysis, genotyping, quantitative real-time RT-PCR enzyme expression analysis | Glycine max |
gene GmUGT4, DNA and amino acid sequence determination and analysis, phylogenetic analysis, genotyping, quantitative real-time RT-PCR enzyme expression analysis | Glycine max |
gene GmUGT7, DNA and amino acid sequence determination and analysis, phylogenetic analysis, genotyping, quantitative real-time RT-PCR enzyme expression analysis | Glycine max |
gene GmUGT9, DNA and amino acid sequence determination and analysis, phylogenetic analysis, genotyping, quantitative real-time RT-PCR enzyme expression analysis | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00031 | - |
genistein | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max | |
0.00042 | - |
daidzein | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max | |
0.0103 | - |
daidzein | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max | |
0.0129 | - |
genistein | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max | |
0.017 | - |
genistein | pH 8.5, 30°C, recombinant isozyme GmUGT1 | Glycine max | |
0.0189 | - |
daidzein | pH 8.5, 30°C, recombinant isozyme GmUGT1 | Glycine max | |
0.0203 | - |
daidzein | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max | |
0.0237 | - |
genistein | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max | |
0.0267 | - |
genistein | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max | |
0.049 | - |
UDP-glucose | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max | |
0.0537 | - |
UDP-glucose | pH 8.5, 30°C, recombinant isozyme GmUGT1 | Glycine max | |
0.0564 | - |
UDP-glucose | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max | |
0.0869 | - |
UDP-glucose | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max | |
0.0985 | - |
daidzein | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max | |
0.1637 | - |
UDP-glucose | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + daidzein | Glycine max | - |
UDP + daidzein 7-O-beta-D-glucoside | i.e. daidzin | ? | |
UDP-glucose + genistein | Glycine max | - |
UDP + genistein 7-O-beta-D-glucoside | i.e. genistin | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
gene GmUGT3 | - |
Glycine max | - |
gene GmUGT4 | - |
Glycine max | - |
gene GmUGT7 | - |
Glycine max | - |
gene GmUGT9 | - |
Glycine max | A6BM07 | gene GmUGT1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | - |
Glycine max | - |
hypocotyl | - |
Glycine max | - |
additional information | gene GmUGT3 encodes a UGT that is highly specific for isoflavones | Glycine max | - |
additional information | gene GmUGT4 encodes a UGT that is highly specific for isoflavones and is mainly expressed in the lateral roots and seeds, followed by taproots, cotyledon, and hypocotyls | Glycine max | - |
additional information | gene GmUGT9 encodes a UGT that is highly specific for isoflavones, the levels of the GmUGT9 transcripts are highest in roots, followed by taproots, cotyledon, and hypocotyls | Glycine max | - |
additional information | the levels of the most abundant GmUGT1 transcripts in cotyledons are much higher than those in lateral roots | Glycine max | - |
additional information | the levels of the most abundant GmUGT7 transcripts in cotyledons are much higher than those in lateral roots | Glycine max | - |
root | lateral | Glycine max | - |
root | lateral and taproot | Glycine max | - |
seed | - |
Glycine max | - |
seedling | - |
Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
GmUGT1 activity with other flavonoids, overview | Glycine max |
0.045 | - |
substrate genistein, pH 8.5, 30°C, purified recombinant enzyme | Glycine max |
0.049 | - |
substrate daidzein, pH 8.5, 30°C, purified recombinant enzyme | Glycine max |
0.158 | - |
substrate genistein, pH 8.5, 30°C, purified recombinant enzyme | Glycine max |
0.209 | - |
substrate genistein, pH 8.5, 30°C, purified recombinant enzyme | Glycine max |
0.253 | - |
substrate daidzein, pH 8.5, 30°C, purified recombinant enzyme | Glycine max |
0.253 | - |
substrate genistein, pH 8.5, 30°C, purified recombinant enzyme | Glycine max |
0.457 | - |
substrate daidzein, pH 8.5, 30°C, purified recombinant enzyme | Glycine max |
0.465 | - |
substrate genistein, pH 8.5, 30°C, purified recombinant enzyme | Glycine max |
0.467 | - |
substrate daidzein, , pH 8.5, 30°C, purified recombinant enzyme | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | GmUGT1 is active with UDP-galactose and UDP-glucuronic acid as substrates. It displays high specific activities toward isoflavones (genistein and daidzein) to produce their 7-O-beta-D-glucosides, it also shows considerable levels of activities toward other flavonoids such as kaempferol, quercetin, naringenin and apigenin. GmUGT1 is also capable of acting on the flavone luteolin, (S)-equol, a 4',7-isoflavandiol, and (R)-equol | Glycine max | ? | - |
? | |
additional information | in contrast to GmUGT1, UDP-galactose and UDP-glucuronic acid are essentially inert as substrates for GmUGT3, glucosyl-acceptor substrate specificity, overview. No activity with kaempferol, quercetin, naringenin, apigenin, and luteolin | Glycine max | ? | - |
? | |
additional information | in contrast to GmUGT1, UDP-galactose and UDP-glucuronic acid are essentially inert as substrates for GmUGT4, glucosyl-acceptor substrate specificity, overview. No activity with kaempferol, quercetin, naringenin, apigenin, and luteolin | Glycine max | ? | - |
? | |
UDP-glucose + daidzein | - |
Glycine max | UDP + daidzein 7-O-beta-D-glucoside | i.e. daidzin | ? | |
UDP-glucose + genistein | - |
Glycine max | UDP + genistein 7-O-beta-D-glucoside | i.e. genistin | ? |
Synonyms | Comment | Organism |
---|---|---|
GmUGT1 | - |
Glycine max |
GmUGT3 | - |
Glycine max |
GmUGT4 | - |
Glycine max |
GmUGT7 | - |
Glycine max |
GmUGT9 | - |
Glycine max |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Glycine max |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
daidzein | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max | |
0.11 | - |
UDP-glucose | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max | |
0.13 | - |
genistein | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max | |
0.39 | - |
genistein | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max | |
0.97 | - |
daidzein | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max | |
1.22 | - |
daidzein | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max | |
1.41 | - |
UDP-glucose | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max | |
1.72 | - |
UDP-glucose | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max | |
1.75 | - |
genistein | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max | |
4.34 | - |
genistein | pH 8.5, 30°C, recombinant isozyme GmUGT1 | Glycine max | |
5.75 | - |
daidzein | pH 8.5, 30°C, recombinant isozyme GmUGT1 | Glycine max | |
5.89 | - |
daidzein | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max | |
6.04 | - |
genistein | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max | |
7.65 | - |
UDP-glucose | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max | |
8.79 | - |
UDP-glucose | pH 8.5, 30°C, recombinant isozyme GmUGT1 | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Glycine max |
General Information | Comment | Organism |
---|---|---|
evolution | nine different GmUGT cDNAs that are related to GmUGT1 are obtained, some of which are classified into two phylogenetically different subgroups, phylogenetic analysis, overview. The enzymes of subgroup A, GmUGT3, GmUGT4, and GmUGT9, all are highly specific for isoflavones. Gene GmUGT4, a representative of subgroup A, encodes a UGT that is highly specific for isoflavones and is mainly expressed in the lateral roots and seeds, while gene GmUGT1, a representative of subgroup B, encodes an enzyme displaying a broad glucosyl-acceptor specificity and is expressed in the aerial parts, i.e. cotyledons, hypocotyls and pods | Glycine max |
evolution | nine different GmUGT cDNAs that are related to GmUGT1, UniProt ID A6BM07, are obtained, some of which are classified into two phylogenetically different subgroups, phylogenetic analysis, overview | Glycine max |
evolution | nine different GmUGT cDNAs that are related to GmUGT1, UniProt ID A6BM07, are obtained, some of which are classified into two phylogenetically different subgroups, phylogenetic analysis, overview. The enzymes of subgroup A, GmUGT3, GmUGT4, and GmUGT9, all are highly specific for isoflavones | Glycine max |
evolution | nine different GmUGT cDNAs that are related to GmUGT1, UniProt ID A6BM07, are obtained, some of which are classified into two phylogenetically different subgroups, phylogenetic analysis, overview. The enzymes of subgroup A, GmUGT3, GmUGT4, and GmUGT9, all are highly specific for isoflavones. Gene GmUGT4, a representative of subgroup A, encodes a UGT that is highly specific for isoflavones and is mainly expressed in the lateral roots and seeds, while gene GmUGT1, a representative of subgroup B, encodes an enzyme displaying a broad glucosyl-acceptor specificity and is expressed in the aerial parts, i.e. cotyledons, hypocotyls and pods | Glycine max |
metabolism | the isoflavone 7-O-glucosyltransferase is involved in isoflavone conjugate biosynthesis | Glycine max |
physiological function | the isoflavone 7-O-glucosyltransferase is involved in isoflavone conjugate biosynthesis. Isoflavone conjugates, 7-O-beta-D-glucosides and 7-O-(6''-malonyl-beta-D-glucosides) of daidzein and genistein, accumulate in soybean roots and serve as the stored precursors of isoflavones (aglycons), which play very important roles in the rhizobia-mediated nodulation of this plant | Glycine max |
physiological function | the isoflavone 7-O-glucosyltransferase is involved in isoflavone conjugate biosynthesis. Isoflavone conjugates, 7-O-beta-D-glucosides and 7-O-(6''-malonyl-beta-D-glucosides) of daidzein and genistein, accumulate in soybean roots and serve as the stored precursors of isoflavones (aglycons), which play very important roles in the rhizobia-mediated nodulation of this plant. Isozyme GmUGT4, but not GmUGT1, plays an exclusive role in the conjugation of isoflavones in the lateral roots of the soybean plant | Glycine max |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
UDP-glucose | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max | |
2 | - |
UDP-glucose | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max | |
10.5 | - |
UDP-glucose | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max | |
88 | - |
UDP-glucose | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max | |
94.8 | - |
daidzein | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max | |
124 | - |
daidzein | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max | |
136 | - |
genistein | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max | |
164 | - |
UDP-glucose | pH 8.5, 30°C, recombinant isozyme GmUGT1 | Glycine max | |
167 | - |
genistein | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max | |
226 | - |
genistein | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max | |
244 | - |
daidzein | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max | |
255 | - |
genistein | pH 8.5, 30°C, recombinant isozyme GmUGT1 | Glycine max | |
291 | - |
daidzein | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max | |
304 | - |
daidzein | pH 8.5, 30°C, recombinant isozyme GmUGT1 | Glycine max | |
409 | - |
genistein | pH 8.5, 30°C, recombinant isozyme GmUGT3 | Glycine max |