Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
eriodictyol | - |
Arabidopsis thaliana | |
0.0263 | - |
kaempferol | - |
Arabidopsis thaliana | |
0.0353 | - |
quercetin | - |
Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q8VZE9 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | AtGT-2 is expressed in this tissue, higher in the flowers and leaves than in roots and stems | Arabidopsis thaliana | - |
leaf | AtGT-2 is expressed in this tissue, higher in the flowers and leaves than in roots and stems | Arabidopsis thaliana | - |
root | AtGT-2 is expressed in this tissue, higher in the flowers and leaves than in roots and stems | Arabidopsis thaliana | - |
stem | AtGT-2 is expressed in this tissue, higher in the flowers and leaves than in roots and stems | Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
Vmax for eriodictyol is 333.3 pkat/mg, 333.3 pkat/mg for kaempferol and 250 pkat/mg for quercetin. Assay at pH 7.0 and 37°C | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | caffeic acid and esculetin do not react with the enzyme. AtGT-2 prefers flavonene to flavone or flavonol, both of which contain a double bond in the C ring. The relative conversion rate is 100% for eriodictyol, 71.6% for naringenin, 29.6% for apigenin, 92.1% for luteolin, 78.9% for kaempferol and 64.1% for quercetin. | Arabidopsis thaliana | ? | - |
? | |
UDP-glucose + eriodictyol | - |
Arabidopsis thaliana | UDP + eriodictyol 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + kaempferol | - |
Arabidopsis thaliana | UDP + kaempferol 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + quercetin | - |
Arabidopsis thaliana | UDP + quercetin 7-O-beta-D-glucoside | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AtGT-2 | AtGT-2 encodes a flavonoid 7-O-glucosyltransferase | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Kcat/Km for eriodictyol is 2.66 microM-1/mg-1, 1.32 microM-1/mg-1 for kaempferol and 250 microM-1/mg-1 for quercetin. Assay at pH 7.0 and 37°C | Arabidopsis thaliana |