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Literature summary for 2.4.1.155 extracted from

  • Song, K.J.; Jeon, S.K.; Moon, S.B.; Park, J.S.; Kim, J.S.; Kim, J.; Kim, S.; An, H.J.; Ko, J.H.; Kim, Y.S.
    Lectin from Sambucus sieboldiana abrogates the anoikis resistance of colon cancer cells conferred by N-acetylglucosaminyltransferase V during hematogenous metastasis (2017), Oncotarget, 8, 42238-42251 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.155

Cloned(Commentary)

Cloned (Comment) Organism
gene expression profiling of cells is conducted by DNA microarray analysis Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information MGAT5 gene knockout in HT-29 cells using CRISPR/Cas9 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus
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 Homo sapiens 5794
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] Homo sapiens
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 UDP + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]
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 ?
UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[TIMP-1] Homo sapiens
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 UDP + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[TIMP-1]
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 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q09328
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Source Tissue

Source Tissue Comment Organism Textmining
colonic cancer cell
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 Homo sapiens
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HT-29 cell
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 Homo sapiens
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LoVo cell
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 Homo sapiens
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additional information transcriptome analysis. Overexpression of the MGAT5 gene has been found in various tumor cells and tissues Homo sapiens
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SW-480 cell
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 Homo sapiens
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WiDr cell
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 Homo sapiens
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]
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 Homo sapiens UDP + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]
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 ?
UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[TIMP-1]
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 Homo sapiens UDP + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[TIMP-1]
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 ?

Synonyms

Synonyms Comment Organism
GnT-V
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 Homo sapiens
Mgat5
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 Homo sapiens
N-acetylglucosaminyltransferase V
-
 Homo sapiens

General Information

General Information Comment Organism
malfunction overexpression of N-acetylglucosaminyltransferase V protects detached cancer cells from apoptotic death, and suppression or knockout of the gene sensitized cancer cells to the apoptotic death. The gene overexpression also stimulates anchorage-dependent as well as anchorage-independent colony formation of cancer cells following anoikis stress treatments Homo sapiens
physiological function N-acetylglucosaminyltransferase V (GnT-V) is a Golgi-located enzyme that catalyzes the branching of the beta1,6-N-acetylglucosamine side chain to the core mannosyl residue of N-linked glycan. N-acetylglucosaminyltransferase V confers anoikis resistance to colon cancer cells during hematogenous metastasis. This resistance is abolished by the lectin from Sambucus sieboldiana, treatment with the lectin significantly sensitizes anoikis-induced cancer cell deaths in vitro as well as in vivo. Anoikis is a form of anchorage-dependent apoptosis, and cancer cells adopt anokis-resistance molecular machinery to conduct metastasis. The MGAT5 gene is a key modulator of anoikis resistance, MGAT5-stimulated anoikis resistance is validated in clinical specimens by correlating the MGAT5 mRNA levels to the cancer stage. MGAT5 potentiates anchorage-dependent and -independent growth following anoikis stress. Glycan profiling identifies unique N-glycan structural features involved in anoikis resistance Homo sapiens