BRENDA - Enzyme Database
show all sequences of 2.4.1.153

Dolichyl phosphate-mannosyltransferase and dolichyl phosphate-N-acetylglucosaminyltransferase activities in liver preparations from normal controls and patients with cystic fibrosis and diabetes mellitus

Alhadeff, J.A.; Watkins P.; Clin. Chim. Acta 134, 1-9 (1983)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Nonidet P-40
detergent required
Homo sapiens
Triton X-100
detergent required
Homo sapiens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mn2+
divalent cation required, 5 mM Mn2+ is optimal
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
Homo sapiens
one of the initial steps of the lipid-mediated glycosylation pathway
UDP + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
normal controls and patients with cystic fibrosis and diabetes mellitus
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
normal controls and patients with cystic fibrosis and diabetes mellitus
Homo sapiens
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
specific activities of dolichyl phosphate-mannosyltransferase are comparable in liver homogenates from normal controls and patients with cystic fibrosis and diabetes mellitus
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
-
488621
Homo sapiens
UDP + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate
-
-
-
?
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
one of the initial steps of the lipid-mediated glycosylation pathway
488621
Homo sapiens
UDP + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
-
Homo sapiens
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Nonidet P-40
detergent required
Homo sapiens
Triton X-100
detergent required
Homo sapiens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mn2+
divalent cation required, 5 mM Mn2+ is optimal
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
Homo sapiens
one of the initial steps of the lipid-mediated glycosylation pathway
UDP + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
normal controls and patients with cystic fibrosis and diabetes mellitus
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
specific activities of dolichyl phosphate-mannosyltransferase are comparable in liver homogenates from normal controls and patients with cystic fibrosis and diabetes mellitus
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
-
488621
Homo sapiens
UDP + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate
-
-
-
?
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
one of the initial steps of the lipid-mediated glycosylation pathway
488621
Homo sapiens
UDP + dolichyl N-acetyl-alpha-D-glucosaminyl phosphate
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
-
Homo sapiens
Other publictions for EC 2.4.1.153
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
732558
Larkin
Biochemical evidence for an al ...
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Nat. Chem. Biol.
9
367-373
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1
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1
-
2
3
1
2
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2
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1
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4
1
1
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1
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1
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1
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2
3
1
2
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1
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4
1
1
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1
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1
1
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660115
Koizumi
-
A chimeric tunicamycin resista ...
Arabidopsis thaliana
Plant Biotechnol.
20
305-309
2003
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1
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1
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488620
Alhadeff
Lipid-mediated glycosylation i ...
Homo sapiens
Enzyme
31
90-103
1984
1
-
-
-
-
-
-
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1
1
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-
1
-
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1
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1
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1
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1
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1
1
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1
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1
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1
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-
-
-
-
-
488621
Alhadeff
Dolichyl phosphate-mannosyltra ...
Homo sapiens
Clin. Chim. Acta
134
1-9
1983
2
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-
-
-
-
-
-
-
1
-
1
-
1
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1
1
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1
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1
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1
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1
1
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2
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1
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