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Literature summary for 2.4.1.15 extracted from

  • Chaudhuri, P.; Basu, A.; Sengupta, S.; Lahiri, S.; Dutta, T.; Ghosh, A.K.
    Studies on substrate specificity and activity regulating factors of trehalose-6-phosphate synthase of Saccharomyces cerevisiae (2009), Biochim. Biophys. Acta, 1790, 368-374.
    View publication on PubMed
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Activating Compound

Activating Compound Comment Organism Structure
CDTA 2.5 mM, 134% of initial activity Saccharomyces cerevisiae
chondroitin stimulation, particularly when a pyrimidine glucose nucleotide is used, rather than a purine glucose nucleotide Saccharomyces cerevisiae
EDTA 2.5 mM, 121% of initial activity Saccharomyces cerevisiae
heparin stimulation, particularly when a pyrimidine glucose nucleotide is used, rather than a purine glucose nucleotide. Heparin helps to retain both stability and activity of the final purified enzyme Saccharomyces cerevisiae
iodoacetamide 1 mM, 245% of initial activity Saccharomyces cerevisiae
iodoacetic acid 0.1 mM, 185% of initial activity Saccharomyces cerevisiae

General Stability

General Stability Organism
heparin helps to retain both stability and activity of the final purified enzyme Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
Citric acid 2.5 mM, 82% residual activity Saccharomyces cerevisiae
N-ethylmaleimide 1 mM, 83% residual activity Saccharomyces cerevisiae
Sodium azide 2.5 mM, 83% residual activity Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
 UDP-glucose pH 8.5, 37°C Saccharomyces cerevisiae
2.17
-
 GDP-glucose pH 8.5, 37°C Saccharomyces cerevisiae
2.38
-
 TDP-glucose pH 8.5, 37°C Saccharomyces cerevisiae
4.5
-
 D-glucose 6-phosphate pH 8.5, 37°C Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
MnCl2 stimulation Saccharomyces cerevisiae
ZnCl2 stimulation Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein protein/sugar ratio is 10.75 Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment) Organism
heparin helps to retain both stability and activity of the final purified enzyme Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.48
-
 pH 8.5, 37°C Saccharomyces cerevisiae
1.58
-
 pH 8.5, 37°C, presence of heparin Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-alpha-D-glucose + D-glucose 6-phosphate
-
 Saccharomyces cerevisiae GDP + alpha,alpha-1,1-trehalose 6-phosphate
-
 ?
TDP-alpha-D-glucose + D-glucose 6-phosphate
-
 Saccharomyces cerevisiae TDP + alpha,alpha-1,1-trehalose 6-phosphate
-
 ?
UDP-alpha-D-glucose + D-fructose 6-phosphate maximum activity with glucose 6-phosphate, followed by mannose 6-phosphate and fructose 6-phosphate Saccharomyces cerevisiae UDP + alpha,alpha-1,1-trehalose 6-phosphate
-
 ?
UDP-alpha-D-glucose + D-glucose 6-phosphate maximum activity with glucose 6-phosphate, followed by mannose 6-phosphate and fructose 6-phosphate Saccharomyces cerevisiae UDP + alpha,alpha-1,1-trehalose 6-phosphate
-
 ?
UDP-alpha-D-glucose + D-mannose 6-phosphate maximum activity with glucose 6-phosphate, followed by mannose 6-phosphate and fructose 6-phosphate Saccharomyces cerevisiae UDP + alpha,alpha-1,1-trehalose 6-phosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
 40 stable within Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Saccharomyces cerevisiae

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5
-
 40 min, 19% residual activity Saccharomyces cerevisiae
8.5
-
 stable at alkaline pH values, 100% stability at pH 8.5 Saccharomyces cerevisiae