Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Glycyrrhiza uralensis |
Protein Variants | Comment | Organism |
---|---|---|
SUS1-DELTA9 | the kcat/Km of the mutant toward sucrose isdecreased by 28.4% compared to that of wild type isoform SUS1, while the kcat/Km of the mutant toward UDP is increased by 1.6% compared to that of SUS1 | Glycyrrhiza uralensis |
SUS2-DELTA9 | the kcat/Km of the mutant toward UDP is increased by 3.8fold compared to that of wild type isoform SUS2, while the kcat/Km of the mutant toward sucrose is decreased by 35.7% compared to that of SUS2 | Glycyrrhiza uralensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
UDP | mutant enzyme SUS2-DELTA9, at pH 5.5 and 50°C | Glycyrrhiza uralensis | |
0.59 | - |
UDP | mutant enzyme SUS1-DELTA9, at pH 5.5 and 50°C | Glycyrrhiza uralensis | |
0.74 | - |
UDP | wild type enzyme isoform SUS1, at pH 5.5 and 50°C | Glycyrrhiza uralensis | |
0.89 | - |
UDP | wild type enzyme isoform SUS2, at pH 5.5 and 50°C | Glycyrrhiza uralensis | |
5.41 | - |
sucrose | wild type enzyme isoform SUS1, at pH 5.5 and 50°C | Glycyrrhiza uralensis | |
9.61 | - |
sucrose | mutant enzyme SUS1-DELTA9, at pH 5.5 and 50°C | Glycyrrhiza uralensis | |
24.47 | - |
sucrose | wild type enzyme isoform SUS2, at pH 5.5 and 50°C | Glycyrrhiza uralensis | |
43.8 | - |
sucrose | mutant enzyme SUS2-DELTA9, at pH 5.5 and 50°C | Glycyrrhiza uralensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NDP-alpha-D-glucose + D-fructose | Glycyrrhiza uralensis | - |
NDP + sucrose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycyrrhiza uralensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Glycyrrhiza uralensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NDP-alpha-D-glucose + D-fructose | - |
Glycyrrhiza uralensis | NDP + sucrose | - |
r | |
UDP + sucrose | - |
Glycyrrhiza uralensis | UDP-alpha-D-glucose + D-fructose | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 90000, SDS-PAGE | Glycyrrhiza uralensis |
Synonyms | Comment | Organism |
---|---|---|
SUS1 | isoform | Glycyrrhiza uralensis |
SUS2 | isoform | Glycyrrhiza uralensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Glycyrrhiza uralensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | isoform SUS1 shows about 40% activity at 30°C, about 68% activity at 40°C, about 80% activity at 45°C, 100% activity at 50°C, about 85% activity at 55°C, and about 35% activity at 60°C. Isoform SUS2 shows about 50% activity at 30°C, about 60% activity at 40°C, about 80% activity at 45°C, 100% activity at 50°C, about 47% activity at 55°C, and about 30% activity at 60°C | Glycyrrhiza uralensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
thermostability of wild type isoforms SUS1 and SUS2 is poor at 50°C. Activity loss occurs rapidly with increasing incubation time, and the half-lives are 50 and 25 min for SUS1 and SUS2, respectively. Mutant enzyme SUS1-DELTA9 shows a half-life increase of 2fold. The residual activity is 50% after 100 min, while the wild type activity remains less than 20% of the original activity. The half-life of the variant SUS2-DELTA9 also increases 2fold | Glycyrrhiza uralensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
50 | - |
UDP | mutant enzyme SUS1-DELTA9, at pH 5.5 and 50°C | Glycyrrhiza uralensis | |
51.2 | - |
sucrose | wild type enzyme isoform SUS1, at pH 5.5 and 50°C | Glycyrrhiza uralensis | |
62 | - |
UDP | wild type enzyme isoform SUS1, at pH 5.5 and 50°C | Glycyrrhiza uralensis | |
74.8 | - |
UDP | wild type enzyme isoform SUS2, at pH 5.5 and 50°C | Glycyrrhiza uralensis | |
76.8 | - |
sucrose | mutant enzyme SUS1-DELTA9, at pH 5.5 and 50°C | Glycyrrhiza uralensis | |
87.1 | - |
sucrose | wild type enzyme isoform SUS2, at pH 5.5 and 50°C | Glycyrrhiza uralensis | |
99.6 | - |
sucrose | mutant enzyme SUS2-DELTA9, at pH 5.5 and 50°C | Glycyrrhiza uralensis | |
121.7 | - |
UDP | mutant enzyme SUS2-DELTA9, at pH 5.5 and 50°C | Glycyrrhiza uralensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Glycyrrhiza uralensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | isoform SUS1 shows about 60% activity at pH 5.0, 100% activity at pH 5.5, about 90% activity at pH 6.5, about 40% activity at pH 7.5, and about 33% activity at pH 8.0. Isoform SUS2 shows about 50% activity at pH 5.0, 100% activity at pH 5.5, about 95% activity at pH 6.5, about 90% activity at pH 7.5, and about 70% activity at pH 8.0 | Glycyrrhiza uralensis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.29 | - |
sucrose | mutant enzyme SUS2-DELTA9, at pH 5.5 and 50°C | Glycyrrhiza uralensis | |
3.56 | - |
sucrose | wild type enzyme isoform SUS2, at pH 5.5 and 50°C | Glycyrrhiza uralensis | |
8.01 | - |
sucrose | mutant enzyme SUS1-DELTA9, at pH 5.5 and 50°C | Glycyrrhiza uralensis | |
11.18 | - |
sucrose | wild type enzyme isoform SUS1, at pH 5.5 and 50°C | Glycyrrhiza uralensis | |
84.3 | - |
UDP | wild type enzyme isoform SUS2, at pH 5.5 and 50°C | Glycyrrhiza uralensis | |
84.42 | - |
UDP | wild type enzyme isoform SUS1, at pH 5.5 and 50°C | Glycyrrhiza uralensis | |
85.75 | - |
UDP | mutant enzyme SUS1-DELTA9, at pH 5.5 and 50°C | Glycyrrhiza uralensis | |
407.83 | - |
UDP | mutant enzyme SUS2-DELTA9, at pH 5.5 and 50°C | Glycyrrhiza uralensis |