BRENDA - Enzyme Database
show all sequences of 2.4.1.13

Sucrose synthase isoforms in cultured tobacco cells

Matic, S.; Akerlund, H.E.; Everitt, E.; Widell, S.; Plant Physiol. Biochem. 42, 299-306 (2004)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
actin
activity of SuSy2 in breakdown direction is stimulated by 60%
Nicotiana tabacum
fructose 2,6-bisphosphate
stimulates SuSy2 in presence of actin
Nicotiana tabacum
Inhibitors
Inhibitors
Commentary
Organism
Structure
actin
0.001 mM, 17% inhibition of SuSy1
Nicotiana tabacum
D-fructose
10 mM, about 80% loss of activity, SuSy1
Nicotiana tabacum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0076
-
UDP
25°C, isoform SuSy1
Nicotiana tabacum
0.032
-
UDP
25°C, isoform SuSy2
Nicotiana tabacum
0.42
-
UDP-glucose
25°C, isoform SuSy1
Nicotiana tabacum
0.45
-
UDP-glucose
25°C, isoform SuSy2
Nicotiana tabacum
1.05
-
D-fructose
25°C, isoform SuSy1
Nicotiana tabacum
2.65
-
D-fructose
25°C, isoform SuSy2
Nicotiana tabacum
22.3
-
sucrose
25°C, isoform SuSy1
Nicotiana tabacum
30.3
-
sucrose
25°C, isoform SuSy2
Nicotiana tabacum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoskeleton
associated to
Nicotiana tabacum
5856
-
cytosol
-
Nicotiana tabacum
5829
-
membrane
bound to
Nicotiana tabacum
16020
-
Organism
Organism
UniProt
Commentary
Textmining
Nicotiana tabacum
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
isoform SuSy1 and SuSy2
Nicotiana tabacum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
UDP + sucrose
-
660272
Nicotiana tabacum
UDP-glucose + D-fructose
-
-
-
r
Synonyms
Synonyms
Commentary
Organism
SuSy
-
Nicotiana tabacum
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.97
-
sucrose
SuSy1, 25°C
Nicotiana tabacum
39.5
-
sucrose
SuSy2, 25°C
Nicotiana tabacum
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
actin
activity of SuSy2 in breakdown direction is stimulated by 60%
Nicotiana tabacum
fructose 2,6-bisphosphate
stimulates SuSy2 in presence of actin
Nicotiana tabacum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
actin
0.001 mM, 17% inhibition of SuSy1
Nicotiana tabacum
D-fructose
10 mM, about 80% loss of activity, SuSy1
Nicotiana tabacum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0076
-
UDP
25°C, isoform SuSy1
Nicotiana tabacum
0.032
-
UDP
25°C, isoform SuSy2
Nicotiana tabacum
0.42
-
UDP-glucose
25°C, isoform SuSy1
Nicotiana tabacum
0.45
-
UDP-glucose
25°C, isoform SuSy2
Nicotiana tabacum
1.05
-
D-fructose
25°C, isoform SuSy1
Nicotiana tabacum
2.65
-
D-fructose
25°C, isoform SuSy2
Nicotiana tabacum
22.3
-
sucrose
25°C, isoform SuSy1
Nicotiana tabacum
30.3
-
sucrose
25°C, isoform SuSy2
Nicotiana tabacum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoskeleton
associated to
Nicotiana tabacum
5856
-
cytosol
-
Nicotiana tabacum
5829
-
membrane
bound to
Nicotiana tabacum
16020
-
Purification (Commentary) (protein specific)
Commentary
Organism
isoform SuSy1 and SuSy2
Nicotiana tabacum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
UDP + sucrose
-
660272
Nicotiana tabacum
UDP-glucose + D-fructose
-
-
-
r
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.97
-
sucrose
SuSy1, 25°C
Nicotiana tabacum
39.5
-
sucrose
SuSy2, 25°C
Nicotiana tabacum
Other publictions for EC 2.4.1.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)