BRENDA - Enzyme Database show
show all sequences of 2.4.1.117

Isolation and characterization of UDP-glucose dolichyl-phosphate glucosyltransferase from human liver

Matern, H.; Bolz, R.; Matern, S.; Eur. J. Biochem. 190, 99-105 (1990)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Phospholipid
required, phosphatidylethanolamine most effective
Homo sapiens
General Stability
General Stability
Organism
uridine or UDPglucuronic acid, 5 mM, protects solubilized enzyme against rapid inactivation
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
UDP
competitive
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Homo sapiens
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36000
-
x * 36000, SDS-PAGE
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Purification (Commentary)
Commentary
Organism
-
Homo sapiens
Renatured (Commentary)
Commentary
Organism
5 mM uridine or UDPglucuronic acid reconstitutes inactivated enzyme
Homo sapiens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-glucose + dolichyl phosphate
-
488484
Homo sapiens
UDP + dolichyl beta-D-glucosyl phosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 36000, SDS-PAGE
Homo sapiens
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Phospholipid
required, phosphatidylethanolamine most effective
Homo sapiens
General Stability (protein specific)
General Stability
Organism
uridine or UDPglucuronic acid, 5 mM, protects solubilized enzyme against rapid inactivation
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
UDP
competitive
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Homo sapiens
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36000
-
x * 36000, SDS-PAGE
Homo sapiens
Purification (Commentary) (protein specific)
Commentary
Organism
-
Homo sapiens
Renatured (Commentary) (protein specific)
Commentary
Organism
5 mM uridine or UDPglucuronic acid reconstitutes inactivated enzyme
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-glucose + dolichyl phosphate
-
488484
Homo sapiens
UDP + dolichyl beta-D-glucosyl phosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 36000, SDS-PAGE
Homo sapiens
Other publictions for EC 2.4.1.117
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
686298
Haecker
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1745-1749
2008
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488488
Arroyo-Flores
Biosynthesis of glycoproteins ...
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488493
Lopez-Romero
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Glycosyl transferases and glyc ...
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Recent Res. Dev. Microbiol.
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667-681
2000
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488492
Rodriguez-Bonilla
Biosynthesis of glycoproteins ...
Candida albicans
Antonie van Leeuwenhoek
73
373-380
1998
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1
1
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3
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488489
Gartung
The submicrosomal localization ...
Homo sapiens
J. Hepatol.
20
32-40
1994
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1
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488491
Heesen
Isolation of the ALG5 locus en ...
Saccharomyces cerevisiae
Eur. J. Biochem.
224
71-79
1994
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1
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488490
Bossuyt
-
Topology of nucleotide-sugar:d ...
Rattus norvegicus
Biochem. J.
296
627-632
1993
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1
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488486
Drake
Partial purificaton, photoaffi ...
Vigna radiata var. radiata
Plant Physiol.
97
396-401
1991
-
-
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-
2
2
1
2
1
1
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3
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1
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1
1
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2
1
2
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488487
Miernyk
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Characterization of maize endo ...
Zea mays
Phytochemistry
30
2865-2867
1991
1
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4
3
1
1
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488484
Matern
Isolation and characterization ...
Homo sapiens
Eur. J. Biochem.
190
99-105
1990
1
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488485
Matern
Control of dolichyl phosphoglu ...
Homo sapiens
Biochim. Biophys. Acta
1004
67-72
1989
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1
4
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488483
Riedell
Glycoprotein synthesis in maiz ...
Zea mays
Plant Physiol.
87
420-426
1988
2
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3
1
1
5
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488480
Villemez
Properties of a soluble polypr ...
Acanthamoeba castellanii
J. Biol. Chem.
254
4814-4819
1979
1
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3
2
1
2
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2
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6
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1
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6
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488481
Herscovics
Glucosyltransferase activity i ...
Bos taurus
J. Biol. Chem.
252
2271-2277
1977
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488482
Behrens
Dolichol monophosphate glucose ...
Rattus norvegicus
Proc. Natl. Acad. Sci. USA
66
153-159
1970
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