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Literature summary for 2.3.3.13 extracted from

  • Ning, J.; Moghe, G.D.; Leong, B.; Kim, J.; Ofner, I.; Wang, Z.; Adams, C.; Jones, A.D.; Zamir, D.; Last, R.L.
    A feedback-insensitive isopropylmalate synthase affects acylsugar composition in cultivated and wild tomato (2015), Plant Physiol., 169, 1821-1835.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Rosetta cells Solanum lycopersicum
expressed in Escherichia coli BL21 Rosetta cells Solanum pennellii

Inhibitors

Inhibitors Comment Organism Structure
L-leucine
-
Solanum lycopersicum
L-leucine
-
Solanum pennellii
additional information isoform IPMS3 is not subject to L-leucine feedback inhibition; isoform IPMS3 is not subject to L-leucine feedback inhibition Solanum lycopersicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.143
-
2-oxoisovalerate isoform IPMS1, pH and temperature not specified in the publication Solanum lycopersicum
0.215
-
2-oxoisovalerate isoform IPMS3, pH and temperature not specified in the publication Solanum lycopersicum
0.734
-
4-methylthio-2-oxobutyrate isoform IPMS3, pH and temperature not specified in the publication Solanum lycopersicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O Solanum lycopersicum
-
(2S)-2-isopropylmalate + CoA
-
?
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O Solanum pennellii
-
(2S)-2-isopropylmalate + CoA
-
?

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum K4C627 isoform IPMS1; M82
-
Solanum lycopersicum K4C627 isoform IPMS2; M82
-
Solanum lycopersicum K4CJ56 isoform IPMS3; M82
-
Solanum pennellii A0A0G2T6D7 isoform IPMS3; LA071
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Solanum lycopersicum
Ni-NTA column chromatography Solanum pennellii

Source Tissue

Source Tissue Comment Organism Textmining
stem
-
Solanum lycopersicum
-
stem
-
Solanum pennellii
-
trichome
-
Solanum lycopersicum
-
trichome
-
Solanum pennellii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 2-oxoisovalerate + H2O
-
Solanum lycopersicum ?
-
?
acetyl-CoA + 2-oxoisovalerate + H2O
-
Solanum pennellii ?
-
?
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O
-
Solanum lycopersicum (2S)-2-isopropylmalate + CoA
-
?
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O
-
Solanum pennellii (2S)-2-isopropylmalate + CoA
-
?
acetyl-CoA + 4-methylthio-2-oxobutyrate + H2O
-
Solanum lycopersicum ?
-
?
acetyl-CoA + 4-methylthio-2-oxobutyrate + H2O
-
Solanum pennellii ?
-
?
additional information isoform IPMS3 shows no activity with 4-methylthio-2-oxobutyrate or 2-oxoisovalerate Solanum pennellii ?
-
?

Synonyms

Synonyms Comment Organism
IPMS1 isoform Solanum lycopersicum
IPMS2 isoform Solanum lycopersicum
IPMS3 isoform Solanum pennellii
IPMS3 isoform Solanum lycopersicum
isopropylmalate synthase
-
Solanum lycopersicum
isopropylmalate synthase
-
Solanum pennellii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.12
-
4-methylthio-2-oxobutyrate isoform IPMS3, pH and temperature not specified in the publication Solanum lycopersicum
0.49
-
2-oxoisovalerate isoform IPMS3, pH and temperature not specified in the publication Solanum lycopersicum
1.08
-
2-oxoisovalerate isoform IPMS1, pH and temperature not specified in the publication Solanum lycopersicum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.16
-
4-methylthio-2-oxobutyrate isoform IPMS3, pH and temperature not specified in the publication Solanum lycopersicum
2.3
-
2-oxoisovalerate isoform IPMS3, pH and temperature not specified in the publication Solanum lycopersicum
7.5
-
2-oxoisovalerate isoform IPMS1, pH and temperature not specified in the publication Solanum lycopersicum