Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Methanocaldococcus jannaschii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-leucine | partially inhibited by L-leucine | Methanocaldococcus jannaschii | |
Mg2+ | inhibitory at 20 mM | Methanocaldococcus jannaschii | |
Mn2+ | inhibitory at 20 mM | Methanocaldococcus jannaschii | |
Zn2+ | inhibitory at 20 mM | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
acetyl-CoA | at pH 7.8 and 37°C | Methanocaldococcus jannaschii | |
0.09 | - |
3-methyl-2-oxobutanoate | at pH 7.8 and 37°C | Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | enzyme activity increases 1.5fold in the presence of 100 mM KCl or NaCl | Methanocaldococcus jannaschii | |
Na+ | enzyme activity increases 1.5fold in the presence of 100 mM KCl or NaCl | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | Methanocaldococcus jannaschii | - |
(2S)-2-isopropylmalate + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+ HisTrap column chromatography | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O | - |
Methanocaldococcus jannaschii | (2S)-2-isopropylmalate + CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Alpha-isopropylmalate synthase | - |
Methanocaldococcus jannaschii |
IPMS | - |
Methanocaldococcus jannaschii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
the enzyme retains full activity after incubation in water for 15 min at up to 90°C | Methanocaldococcus jannaschii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
330 | - |
3-methyl-2-oxobutanoate | at pH 7.8 and 37°C | Methanocaldococcus jannaschii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
L-leucine | at pH 7.8 and 37 °C | Methanocaldococcus jannaschii |