Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.3.13 extracted from

  • Casey, A.K.; Hicks, M.A.; Johnson, J.L.; Babbitt, P.C.; Frantom, P.A.
    Mechanistic and bioinformatic investigation of a conserved active site helix in alpha-isopropylmalate synthase from Mycobacterium tuberculosis, a member of the DRE-TIM metallolyase superfamily (2014), Biochemistry, 53, 2915-2925.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Methanocaldococcus jannaschii

Inhibitors

Inhibitors Comment Organism Structure
L-leucine partially inhibited by L-leucine Methanocaldococcus jannaschii
Mg2+ inhibitory at 20 mM Methanocaldococcus jannaschii
Mn2+ inhibitory at 20 mM Methanocaldococcus jannaschii
Zn2+ inhibitory at 20 mM Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
acetyl-CoA at pH 7.8 and 37°C Methanocaldococcus jannaschii
0.09
-
3-methyl-2-oxobutanoate at pH 7.8 and 37°C Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions Comment Organism Structure
K+ enzyme activity increases 1.5fold in the presence of 100 mM KCl or NaCl Methanocaldococcus jannaschii
Na+ enzyme activity increases 1.5fold in the presence of 100 mM KCl or NaCl Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O Methanocaldococcus jannaschii
-
(2S)-2-isopropylmalate + CoA
-
?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+ HisTrap column chromatography Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O
-
Methanocaldococcus jannaschii (2S)-2-isopropylmalate + CoA
-
?

Synonyms

Synonyms Comment Organism
Alpha-isopropylmalate synthase
-
Methanocaldococcus jannaschii
IPMS
-
Methanocaldococcus jannaschii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
the enzyme retains full activity after incubation in water for 15 min at up to 90°C Methanocaldococcus jannaschii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
330
-
3-methyl-2-oxobutanoate at pH 7.8 and 37°C Methanocaldococcus jannaschii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.16
-
L-leucine at pH 7.8 and 37 °C Methanocaldococcus jannaschii