Protein Variants | Comment | Organism |
---|---|---|
D546A | the enzyme shows wild type activity but is not inhibited by L-leucine | Mycobacterium tuberculosis |
D563N | the enzyme shows wild type activity but is not inhibited by L-leucine | Mycobacterium tuberculosis |
Y410F | the mutant is insensitive to L-leucine inhibition despite retaining significant binding affinity for the molecule | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-isoleucine | noncompetitive inhibitor | Mycobacterium tuberculosis | |
L-leucine | noncompetitive inhibitor versus 2-oxo-3-methylbutanoate | Mycobacterium tuberculosis | |
L-norleucine | noncompetitive inhibitor | Mycobacterium tuberculosis | |
L-norvaline | noncompetitive inhibitor | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | Co2+ is capable of supporting robust catalytic activity | Mycobacterium tuberculosis | |
K+ | K+ is the kinetically preferred ion | Mycobacterium tuberculosis | |
Mg2+ | Mg2+ is capable of supporting robust catalytic activity | Mycobacterium tuberculosis | |
Mn2+ | Mn2+ is capable of supporting robust catalytic activity | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
144000 | - |
- |
Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | the enzyme catalyzes a Claisen-condensation between acetyl-CoA and 2-oxo-3-methylbutanoate (alpha-ketoisovalerate) followed by hydrolysis to form 3-hydroxy-4-methyl-3-carboxypentanoate (alpha-isopropylmalate) and CoA | Mycobacterium tuberculosis | 3-hydroxy-4-methyl-3-carboxypentanoate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
alpha-isopropylmalate synthase I | - |
Mycobacterium tuberculosis |
IPMS | - |
Mycobacterium tuberculosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
L-leucine | pH and temperature not specified in the publication | Mycobacterium tuberculosis |