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Literature summary for 2.3.3.13 extracted from

  • Frantom, P.A.; Zhang, H.M.; Emmett, M.R.; Marshall, A.G.; Blanchard, J.S.
    Mapping of the allosteric network in the regulation of alpha-isopropylmalate synthase from Mycobacterium tuberculosis by the feedback inhibitor L-leucine: solution-phase H/D exchange monitored by FT-ICR mass spectrometry (2009), Biochemistry, 48, 7457-7464.
    View publication on PubMed

Application

Application Comment Organism
medicine alpha-isopropylmalate synthase is a target for the design of new antitubercular therapeutics Mycobacterium tuberculosis

Cloned(Commentary)

Cloned (Comment) Organism
-
Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
Y410F mutant, insensitive to L-leucine inhibition Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
L-leucine feedback inhibition Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O Mycobacterium tuberculosis
-
(2S)-2-isopropylmalate + CoA
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
on a nickel-NTA, a Mono-Q and a Phenyl Sepharose column Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 3-methyl-2-oxobutanoate + H2O
-
Mycobacterium tuberculosis (2S)-2-isopropylmalate + CoA
-
?
alpha-ketoisovaleric acid + H2O + acetyl-CoA
-
Mycobacterium tuberculosis ? + CoA
-
?

Synonyms

Synonyms Comment Organism
Alpha-isopropylmalate synthase
-
Mycobacterium tuberculosis
MtIPMS
-
Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
acetyl-CoA
-
Mycobacterium tuberculosis