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Literature summary for 2.3.3.13 extracted from

  • Wiegel, J.
    alpha-Isopropylmalate synthase as a marker for the leucine biosynthetic pathway in several clostridia and in Bacteroides fragilis (1981), Arch. Microbiol., 130, 385-390.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-Oxo-isohexanoate 1 mM Bacteroides fragilis
2-Oxo-isohexanoate 1 mM Clostridium formicaceticum
2-Oxo-isohexanoate 1 mM Clostridium kluyveri
2-Oxo-isohexanoate 1 mM Clostridium pasteurianum
2-Oxo-isohexanoate 1 mM Moorella thermoacetica
EDTA time-dependent inactivation is not reversible by intensive dialysis Clostridium pasteurianum
leucine
-
Bacteroides fragilis
leucine
-
Clostridium formicaceticum
leucine
-
Clostridium pasteurianum
leucine
-
Moorella thermoacetica

Metals/Ions

Metals/Ions Comment Organism Structure
additional information enzyme contains a metal ion Clostridium pasteurianum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-oxo-3-methylbutanoate + acetyl-CoA + H2O Moorella thermoacetica repression by Leu ?
-
?
2-oxo-3-methylbutanoate + acetyl-CoA + H2O Moorella thermoacetica involved in Leu biosynthesis ?
-
?
2-oxo-3-methylbutanoate + acetyl-CoA + H2O Bacteroides fragilis involved in Leu biosynthesis ?
-
?

Organism

Organism UniProt Comment Textmining
Bacteroides fragilis
-
-
-
Clostridium formicaceticum
-
-
-
Clostridium kluyveri
-
-
-
Clostridium pasteurianum
-
-
-
Moorella thermoacetica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Clostridium pasteurianum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0019
-
-
Clostridium pasteurianum
0.0024
-
-
Clostridium formicaceticum
0.0086
-
-
Bacteroides fragilis
0.0089
-
-
Moorella thermoacetica
1.413
-
-
Clostridium pasteurianum

Storage Stability

Storage Stability Organism
-18°C, 50 mM potassium phosphate, pH 7.2, loss of activity after 3 months Clostridium pasteurianum
0°C, more than 80% loss of activity after 2 d Clostridium pasteurianum
22°C, in presence of 0.05% sodium azide, 8% loss of activity after 3 months Clostridium pasteurianum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxo-3-methylbutanoate + acetyl-CoA + H2O
-
Moorella thermoacetica 3-hydroxy-4-methyl-3-carboxypentanoate + CoA
-
?
2-oxo-3-methylbutanoate + acetyl-CoA + H2O
-
Bacteroides fragilis 3-hydroxy-4-methyl-3-carboxypentanoate + CoA
-
?
2-oxo-3-methylbutanoate + acetyl-CoA + H2O
-
Clostridium pasteurianum 3-hydroxy-4-methyl-3-carboxypentanoate + CoA
-
?
2-oxo-3-methylbutanoate + acetyl-CoA + H2O
-
Clostridium kluyveri 3-hydroxy-4-methyl-3-carboxypentanoate + CoA
-
?
2-oxo-3-methylbutanoate + acetyl-CoA + H2O
-
Clostridium formicaceticum 3-hydroxy-4-methyl-3-carboxypentanoate + CoA
-
?
2-oxo-3-methylbutanoate + acetyl-CoA + H2O repression by Leu Moorella thermoacetica ?
-
?
2-oxo-3-methylbutanoate + acetyl-CoA + H2O involved in Leu biosynthesis Moorella thermoacetica ?
-
?
2-oxo-3-methylbutanoate + acetyl-CoA + H2O involved in Leu biosynthesis Bacteroides fragilis ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37 40
-
Clostridium pasteurianum
46
-
-
Moorella thermoacetica
46
-
-
Clostridium formicaceticum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Bacteroides fragilis
8.5
-
-
Clostridium pasteurianum
8.5
-
-
Clostridium kluyveri
8.8
-
-
Moorella thermoacetica
8.8
-
-
Clostridium formicaceticum