Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Oxo-isohexanoate | 1 mM | Bacteroides fragilis | |
2-Oxo-isohexanoate | 1 mM | Clostridium formicaceticum | |
2-Oxo-isohexanoate | 1 mM | Clostridium kluyveri | |
2-Oxo-isohexanoate | 1 mM | Clostridium pasteurianum | |
2-Oxo-isohexanoate | 1 mM | Moorella thermoacetica | |
EDTA | time-dependent inactivation is not reversible by intensive dialysis | Clostridium pasteurianum | |
leucine | - |
Bacteroides fragilis | |
leucine | - |
Clostridium formicaceticum | |
leucine | - |
Clostridium pasteurianum | |
leucine | - |
Moorella thermoacetica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme contains a metal ion | Clostridium pasteurianum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | Moorella thermoacetica | repression by Leu | ? | - |
? | |
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | Moorella thermoacetica | involved in Leu biosynthesis | ? | - |
? | |
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | Bacteroides fragilis | involved in Leu biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteroides fragilis | - |
- |
- |
Clostridium formicaceticum | - |
- |
- |
Clostridium kluyveri | - |
- |
- |
Clostridium pasteurianum | - |
- |
- |
Moorella thermoacetica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Clostridium pasteurianum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0019 | - |
- |
Clostridium pasteurianum |
0.0024 | - |
- |
Clostridium formicaceticum |
0.0086 | - |
- |
Bacteroides fragilis |
0.0089 | - |
- |
Moorella thermoacetica |
1.413 | - |
- |
Clostridium pasteurianum |
Storage Stability | Organism |
---|---|
-18°C, 50 mM potassium phosphate, pH 7.2, loss of activity after 3 months | Clostridium pasteurianum |
0°C, more than 80% loss of activity after 2 d | Clostridium pasteurianum |
22°C, in presence of 0.05% sodium azide, 8% loss of activity after 3 months | Clostridium pasteurianum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | - |
Moorella thermoacetica | 3-hydroxy-4-methyl-3-carboxypentanoate + CoA | - |
? | |
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | - |
Bacteroides fragilis | 3-hydroxy-4-methyl-3-carboxypentanoate + CoA | - |
? | |
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | - |
Clostridium pasteurianum | 3-hydroxy-4-methyl-3-carboxypentanoate + CoA | - |
? | |
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | - |
Clostridium kluyveri | 3-hydroxy-4-methyl-3-carboxypentanoate + CoA | - |
? | |
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | - |
Clostridium formicaceticum | 3-hydroxy-4-methyl-3-carboxypentanoate + CoA | - |
? | |
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | repression by Leu | Moorella thermoacetica | ? | - |
? | |
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | involved in Leu biosynthesis | Moorella thermoacetica | ? | - |
? | |
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | involved in Leu biosynthesis | Bacteroides fragilis | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 40 | - |
Clostridium pasteurianum |
46 | - |
- |
Moorella thermoacetica |
46 | - |
- |
Clostridium formicaceticum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Bacteroides fragilis |
8.5 | - |
- |
Clostridium pasteurianum |
8.5 | - |
- |
Clostridium kluyveri |
8.8 | - |
- |
Moorella thermoacetica |
8.8 | - |
- |
Clostridium formicaceticum |