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Literature summary for 2.3.3.13 extracted from

  • Beltzer, J.P.; Morris, S.R.; Kohlhaw, G.B.
    Yeast LEU4 encodes mitochondrial and nonmitochondrial forms of alpha-isopropylmalate synthase (1988), J. Biol. Chem., 263, 368-374.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
construction of a Leu4'-'lacZ fusion. The Leu4 gene encodes 2 forms: a short cytoplasmic form and a long form that is targeted to the mitochondria Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
leucine
-
Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm the Leu4 gene encodes 2 forms: a short cytoplasmic form and a long form that is targeted to the mitochondria Saccharomyces cerevisiae 5737
-
mitochondrion the Leu4 gene encodes 2 forms: a short cytoplasmic form and a long form that is targeted to the mitochondria Saccharomyces cerevisiae 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
2 * 65000, cytoplasmic enzyme Saccharomyces cerevisiae
130000
-
cytoplasmic form, gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-oxo-3-methylbutanoate + acetyl-CoA + H2O Saccharomyces cerevisiae the cytoplasmic form is involved in Leu biosynthesis ?
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
the Leu4 gene encodes 2 forms: a short cytoplasmic form and a long form that is targeted to the mitochondria
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxo-3-methylbutanoate + acetyl-CoA + H2O
-
Saccharomyces cerevisiae 3-hydroxy-4-methyl-3-carboxypentanoate + CoA i.e. alpha-isopropylmalate ?
2-oxo-3-methylbutanoate + acetyl-CoA + H2O the cytoplasmic form is involved in Leu biosynthesis Saccharomyces cerevisiae ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 65000, cytoplasmic enzyme Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.4
-
leucine
-
Saccharomyces cerevisiae