Cloned (Comment) | Organism |
---|---|
construction of a Leu4'-'lacZ fusion. The Leu4 gene encodes 2 forms: a short cytoplasmic form and a long form that is targeted to the mitochondria | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
leucine | - |
Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | the Leu4 gene encodes 2 forms: a short cytoplasmic form and a long form that is targeted to the mitochondria | Saccharomyces cerevisiae | 5737 | - |
mitochondrion | the Leu4 gene encodes 2 forms: a short cytoplasmic form and a long form that is targeted to the mitochondria | Saccharomyces cerevisiae | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
2 * 65000, cytoplasmic enzyme | Saccharomyces cerevisiae |
130000 | - |
cytoplasmic form, gel filtration | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | Saccharomyces cerevisiae | the cytoplasmic form is involved in Leu biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
the Leu4 gene encodes 2 forms: a short cytoplasmic form and a long form that is targeted to the mitochondria | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | - |
Saccharomyces cerevisiae | 3-hydroxy-4-methyl-3-carboxypentanoate + CoA | i.e. alpha-isopropylmalate | ? | |
2-oxo-3-methylbutanoate + acetyl-CoA + H2O | the cytoplasmic form is involved in Leu biosynthesis | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 65000, cytoplasmic enzyme | Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
leucine | - |
Saccharomyces cerevisiae |