Literature summary for 2.3.2.31 extracted from

Pao, K.C.; Stanley, M.; Han, C.; Lai, Y.C.; Murphy, P.; Balk, K.; Wood, N.T.; Corti, O.; Corvol, J.C.; Muqit, M.M.; Virdee, S.
Probes of ubiquitin E3 ligases enable systematic dissection of parkin activation (2016), Nat. Chem. Biol., 12, 324-331 .

Search for more literature for 2.3.2.31

Application

Application	Comment	Organism
analysis	development of activity-based probes by reengineering of a ubiquitin-charged E2 conjugating enzyme using tosyl-substituted doubly activated genes and application in profiling the transthiolation activity of the RBR E3 ligase Parkin in vitro and in cellular extracts	Homo sapiens
medicine	parkin patient disease-associated mutation largely mediate their effect by altering transthiolation activity	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
A46P	parkin disease mutant, loss of activity	Homo sapiens
C431F	parkin disease mutant, loss of activity	Homo sapiens
G430D	parkin disease mutant, loss of activity	Homo sapiens
K161N	parkin disease mutant, loss of activity	Homo sapiens
K211N	parkin disease mutant, loss of activity	Homo sapiens
K27N	parkin disease mutant, 0.7fold reduction in activity	Homo sapiens
R275W	parkin disease mutant, loss of activity	Homo sapiens
R42P	parkin disease mutant, 2.2fold activation of activity	Homo sapiens
S65A	parkin disease mutant, loss of activity	Homo sapiens
T415N	parkin disease mutant, loss of activity	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	O60260	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
neuron	endogenous Parkin is activated in neuronal cell lines in response to mitochondrial depolarization	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
[E2 ubiquitin-conjugating enzyme UBE2L3]-S-ubiquitinyl-L-cysteine + [Miro1]-L-lysine	-	Homo sapiens	[E2 ubiquitin-conjugating enzyme UBE2L3]-L-cysteine + [Miro1]-N6-ubiquitinyl-L-lysine	-	?

Synonyms

Synonyms	Comment	Organism
Parkin	-	Homo sapiens

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Release 2023.1 (January 2023)