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Literature summary for 2.3.2.23 extracted from

  • David, Y.; Ziv, T.; Admon, A.; Navon, A.
    The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines (2010), J. Biol. Chem., 285, 8595-8604.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed and transformed into Escherichia coli (BL-21 DE3) using pET22 vector in frame to a C-terminal His6 tag Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein, Ni-NTA column Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine E2 enzymes autoubiquitinate both their active sites cysteine, described for this enzyme and lysine residues Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information polyubiquitin chain formation catalyzed by E2 enzymes, in the absence of an E3 protein and a target protein substrate Homo sapiens ? a thiol ester-linked ubiquitin to the E2 active site is an intermediate in any polyubiquination reactions ?

Subunits

Subunits Comment Organism
dimer E2 enzymes spontaneously dimerize in solution, in vitro, in absence of charged ubiquitin Homo sapiens

Synonyms

Synonyms Comment Organism
E2 ubiquitin-conjugating enzyme
-
Homo sapiens

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
37
-
assay at, polyubiquitination Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at, polyubiquitination Homo sapiens