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Literature summary for 2.3.2.13 extracted from

  • Deweid, L.; Avrutina, O.; Kolmar, H.
    Microbial transglutaminase for biotechnological and biomedical engineering (2019), Biol. Chem., 400, 257-274 .
    View publication on PubMed
Search for more literature for 2.3.2.13

Application

Application Comment Organism
biotechnology the microbial transglutaminase is used for biotechnological and biomedical engineering, protein engineering by post-translational modification towards the generation of multifunctional conjugates. Biotechnological applications, detailed overview. Transglutaminase-mediated surface immobilization, a widely-used technique to increase stability of labile and cost-intensive enzymes and enable their reuse Streptomyces mobaraensis
medicine the microbial transglutaminase is used for biotechnological and biomedical engineering, protein engineering by post-translational modification towards the generation of multifunctional conjugates. Biotechnological applications, detailed overview. Construction of protein-polymer and of antibody-drug conjugations for pharmaceutical applications Streptomyces mobaraensis

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure analysis, PDB ID 4P8I Bacillus subtilis
crystal structure analysis, PDB ID 5M6Q Kutzneria albida
crystal structure analysis, PDB IDs 3IU0 and 1IU4 Streptomyces mobaraensis

Protein Variants

Protein Variants Comment Organism
additional information peptidyl-linker sequences used that facilitate modification by microbial transglutaminase, overview Streptomyces mobaraensis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm immature pro-enzyme Streptomyces mobaraensis 5737
-
extracellular the immature enzyme is secreted, and extracellulary, the zymogen is processed by an endogenous metalloprotease called TGase-activating protease (TAMEP) and a tripeptidyl aminopeptidase (SM-TAP) Streptomyces mobaraensis
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Streptomyces mobaraensis
Ca2+ required Kutzneria albida
Ca2+ required Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protein glutamine + alkylamine Streptomyces mobaraensis
-
 protein N5-alkylglutamine + NH3
-
 ?
protein glutamine + alkylamine Kutzneria albida
-
 protein N5-alkylglutamine + NH3
-
 ?
protein glutamine + alkylamine Bacillus subtilis
-
 protein N5-alkylglutamine + NH3
-
 ?
protein glutamine + alkylamine Bacillus subtilis 168
-
 protein N5-alkylglutamine + NH3
-
 ?
protein glutamine + alkylamine Kutzneria albida DSM 43870
-
 protein N5-alkylglutamine + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P40746
-
-
Bacillus subtilis 168 P40746
-
-
Kutzneria albida W5WHY8
-
-
Kutzneria albida DSM 43870 W5WHY8
-
-
Streptomyces mobaraensis P81453
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the immature enzyme is secreted, and extracellulary, the zymogen is processed by an endogenous metalloprotease called TGase-activating protease (TAMEP) and a tripeptidyl aminopeptidase (SM-TAP). SM-TAP procession is not essential for activity as TAMEP-treated and fully processed zymogen exhibits similar catalytic activity Streptomyces mobaraensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information SM-TAP procession of the pro-form zymogen is not essential for activity as TAMEP-treated and fully processed enzyme and the zymogen exhibit similar catalytic activity Streptomyces mobaraensis ?
-
-
protein glutamine + alkylamine
-
 Streptomyces mobaraensis protein N5-alkylglutamine + NH3
-
 ?
protein glutamine + alkylamine
-
 Kutzneria albida protein N5-alkylglutamine + NH3
-
 ?
protein glutamine + alkylamine
-
 Bacillus subtilis protein N5-alkylglutamine + NH3
-
 ?
protein glutamine + alkylamine
-
 Bacillus subtilis 168 protein N5-alkylglutamine + NH3
-
 ?
protein glutamine + alkylamine
-
 Kutzneria albida DSM 43870 protein N5-alkylglutamine + NH3
-
 ?

Subunits

Subunits Comment Organism
? x * 28000 Bacillus subtilis
? x * 26400 Kutzneria albida
monomer 1 * 37900, mature enzyme, 1 * 42500, enzyme pro-form Streptomyces mobaraensis

Synonyms

Synonyms Comment Organism
KALB
-
 Kutzneria albida
KALB_7456
-
 Kutzneria albida
microbial transglutaminase
-
 Streptomyces mobaraensis
microbial transglutaminase
-
 Kutzneria albida
microbial transglutaminase
-
 Bacillus subtilis
TGL
-
 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Streptomyces mobaraensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7
-
 Streptomyces mobaraensis

pI Value

Organism Comment pI Value Maximum pI Value
Streptomyces mobaraensis
-
-
 8

General Information

General Information Comment Organism
additional information mechanism of mTG-catalyzed isopeptide bond formation between protein-bound glutamine and lysine residues. Structure-function analysis, substrate specificity, overview Streptomyces mobaraensis
additional information mechanism of mTG-catalyzed isopeptide bond formation between protein-bound glutamine and lysine residues. Structure-function analysis, substrate specificity, overview Kutzneria albida
additional information mechanism of mTG-catalyzed isopeptide bond formation between protein-bound glutamine and lysine residues. Structure-function analysis, substrate specificity, overview Bacillus subtilis