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Literature summary for 2.3.1.B43 extracted from

  • Du, Y.; Hu, H.; Qu, S.; Wang, J.; Hua, C.; Zhang, J.; Wei, P.; He, X.; Hao, J.; Liu, P.; Yang, F.; Li, T.; Wei, T.
    SIRT5 deacylates metabolism-related proteins and attenuates hepatic steatosis in ob/ob mice (2018), EBioMedicine, 36, 347-357 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.B43

Cloned(Commentary)

Cloned (Comment) Organism
gene SIRT5, genotyping of the Ctl and OE mice. Sirt5 hepatic overexpressing (ob/ob-SIRT5 OE) mouse containing at least one allele of alternative Sirt5 gene and Alb-cre. The other one is control ob/ob mouse (ob/ob- control) containing only the floxed Sirt5 KI allele Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information hepatic SIRT5-overexpressing ob/ob mouse model (ob/ob-SIRT5 OE) is established by CRISPR/Cas9 gene editing tool. Analysis of commercially available Sirt5 conditional knock-in C57BL/6 mice. Identification, quantification, and analysis of the malonylome and succinylome in response to hepatic overexpression of SIRT5 in ob/ob mice. Hepatic overexpression of SIRT5 in ob/ob mice demalonylates proteins in the glycolysis/gluconeogenesis pathway to improve glucose metabolism, and desuccinylates proteins in the oxidative phosphorylation pathway to facilitate fatty acid metabolism Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Mus musculus 5739
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Organism

Organism UniProt Comment Textmining
Mus musculus A0A1Y7VM56
-
-
Mus musculus C57BL/6 A0A1Y7VM56
-
-

Source Tissue

Source Tissue Comment Organism Textmining
adipose tissue
-
 Mus musculus
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liver
-
 Mus musculus
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skeletal muscle
-
 Mus musculus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information protein malonylation and succinylation lysine sites are identified by immunoprecipitation coupled lipid chromatography-tandem mass spectrometry (LC-MS/MS) methods Mus musculus ?
-
-
additional information protein malonylation and succinylation lysine sites are identified by immunoprecipitation coupled lipid chromatography-tandem mass spectrometry (LC-MS/MS) methods Mus musculus C57BL/6 ?
-
-

Synonyms

Synonyms Comment Organism
SIRT5
-
 Mus musculus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Mus musculus

General Information

General Information Comment Organism
malfunction the SIRT5 deficiency mouse model shows that it is dispensable for metabolic homeostasis under normal conditions. The ob/ob-SIRT5 OE mice show decreased malonylation and succinylation, improved cellular glycolysis, suppressed gluconeogenesis, enhanced fatty acid oxidation, and attenuated hepatic steatosis. Hepatic overexpression of SIRT5 ameliorates the metabolic abnormalities of ob/obmice, probably through demalonylating and desuccinylating proteins in the main metabolic pathways. SIRT5 and related acylation might be potential targets for metabolic disorders Mus musculus
metabolism protein malonylation and succinylation lysine sites are identified by immunoprecipitation coupled lipid chromatography-tandem mass spectrometry (LC-MS/MS) methods. A total of 955 malonylation sites on 434 proteins and 1377 succinylation sites on 429 proteins were identified and quantitated. Malonylation is the major SIRT5 target in the glycolysis/gluconeogenesis pathway, whereas succinylation is the preferred SIRT5 target in the oxidative phosphorylation pathway. Identification, quantification, and analysis of malonylome and succinylome Mus musculus
physiological function Sirtuin 5 (SIRT5) is a NAD+-dependent lysine deacylase. SIRT5 deacylates metabolism-related proteins and attenuates hepatic steatosis in obese ob/ob mice Mus musculus