BRENDA - Enzyme Database
show all sequences of 2.3.1.B41

Plasmodium falciparum Sir2: an unusual sirtuin with dual histone deacetylase and ADP-ribosyltransferase activity

Merrick, C.J.; Duraisingh, M.T.; Eukaryot. Cell 6, 2081-291 (2007)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
the enzyme is unaffected by the activators isonicotinamide and resveratrol
Plasmodium falciparum
Application
Application
Commentary
Organism
medicine
PfSir2A inhibitors may have therapeutic value in malaria treatment
Plasmodium falciparum
Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli with a C-terminal His6 tag and an N-terminal T7 tag
Plasmodium falciparum
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
splitomicin and sirtinol fail to inhibit PfSir2
Plasmodium falciparum
nicotinamide
inhibits the deacetylation of native histones much more effectively than deacetylation of a synthetic substrate
Plasmodium falciparum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
x * 30000, SDS-PAGE
Plasmodium falciparum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Plasmodium falciparum
Q8IE47
-
-
Plasmodium falciparum isolate 3D7
Q8IE47
-
-
Purification (Commentary)
Commentary
Organism
-
Plasmodium falciparum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
NAD+ + [histone H3]-N6-acetyl-L-lysine
-
729665
Plasmodium falciparum
nicotinamide + [histone H3]-L-lysine + 2'-O-acetyl-ADP-ribose
-
-
-
?
NAD+ + [histone H3]-N6-acetyl-L-lysine
-
729665
Plasmodium falciparum isolate 3D7
nicotinamide + [histone H3]-L-lysine + 2'-O-acetyl-ADP-ribose
-
-
-
?
NAD+ + [histone H4]-N6-acetyl-L-lysine
-
729665
Plasmodium falciparum
nicotinamide + [histone H4]-L-lysine + 2'-O-acetyl-ADP-ribose
-
-
-
?
NAD+ + [histone H4]-N6-acetyl-L-lysine
-
729665
Plasmodium falciparum isolate 3D7
nicotinamide + [histone H4]-L-lysine + 2'-O-acetyl-ADP-ribose
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 30000, SDS-PAGE
Plasmodium falciparum
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Plasmodium falciparum
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
the enzyme is unaffected by the activators isonicotinamide and resveratrol
Plasmodium falciparum
Application (protein specific)
Application
Commentary
Organism
medicine
PfSir2A inhibitors may have therapeutic value in malaria treatment
Plasmodium falciparum
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli with a C-terminal His6 tag and an N-terminal T7 tag
Plasmodium falciparum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
splitomicin and sirtinol fail to inhibit PfSir2
Plasmodium falciparum
nicotinamide
inhibits the deacetylation of native histones much more effectively than deacetylation of a synthetic substrate
Plasmodium falciparum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
30000
-
x * 30000, SDS-PAGE
Plasmodium falciparum
Purification (Commentary) (protein specific)
Commentary
Organism
-
Plasmodium falciparum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
NAD+ + [histone H3]-N6-acetyl-L-lysine
-
729665
Plasmodium falciparum
nicotinamide + [histone H3]-L-lysine + 2'-O-acetyl-ADP-ribose
-
-
-
?
NAD+ + [histone H3]-N6-acetyl-L-lysine
-
729665
Plasmodium falciparum isolate 3D7
nicotinamide + [histone H3]-L-lysine + 2'-O-acetyl-ADP-ribose
-
-
-
?
NAD+ + [histone H4]-N6-acetyl-L-lysine
-
729665
Plasmodium falciparum
nicotinamide + [histone H4]-L-lysine + 2'-O-acetyl-ADP-ribose
-
-
-
?
NAD+ + [histone H4]-N6-acetyl-L-lysine
-
729665
Plasmodium falciparum isolate 3D7
nicotinamide + [histone H4]-L-lysine + 2'-O-acetyl-ADP-ribose
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 30000, SDS-PAGE
Plasmodium falciparum
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Plasmodium falciparum
Other publictions for EC 2.3.1.B41
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737930
Cea
Evidence for a role of the his ...
Homo sapiens
Blood
127
1138-1150
2016
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2
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738060
Rahnasto-Rilla
N-acylethanolamines bind to SI ...
Homo sapiens
ChemBioChem
17
77-81
2016
12
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1
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739139
Zhang
Identifying the functional con ...
Homo sapiens
Nat. Chem. Biol.
12
614-620
2016
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4
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-
-
-
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1
1
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739158
Tasselli
SIRT6 deacetylates H3K18Ac at ...
Homo sapiens
Nat. Struct. Mol. Biol.
23
434-440
2016
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2
2
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739225
Liu
Cyclic peptide-based potent hu ...
Homo sapiens
Org. Biomol. Chem.
14
5928-5935
2016
-
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2
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1
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2
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730473
Cardinale
Biochemical characterization o ...
Mus musculus
Neurochem. Res.
40
59-69
2015
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1
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1
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1
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737946
Hwang
SIRT6 protein deacetylase inte ...
Mus musculus
BMC Mol. Biol.
16
12
2015
-
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1
-
1
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737977
Feng
Sirtuin 6 promotes transformin ...
Homo sapiens
Cancer Sci.
106
559-566
2015
-
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738169
Zhang
The histone deacetylase SIRT6 ...
Homo sapiens
Eur. Rev. Med. Pharmacol. Sci.
19
818-824
2015
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1
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1
1
1
1
-
-
738170
Maksin-Matveev
Sirtuin 6 protects the heart f ...
Mus musculus
Exp. Cell Res.
330
81-90
2015
-
-
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-
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3
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1
1
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738283
Hu
Posttranslational modification ...
Homo sapiens
Free Radic. Biol. Med.
79
176-185
2015
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1
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4
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1
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2
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1
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1
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739132
Etchegaray
The histone deacetylase SIRT6 ...
Mus musculus
Nat. Cell Biol.
17
545-557
2015
-
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2
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2
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739733
Tian
Sirtuin-6 inhibits cardiac fib ...
Rattus norvegicus
Transl. Res.
165
374-386
2015
-
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1
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1
2
2
1
-
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729145
Chen
The histone deacetylase SIRT6 ...
Homo sapiens
Biochem. Biophys. Res. Commun.
446
364-369
2014
-
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1
1
1
1
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729362
Liu
Oxidative stress induces endot ...
Homo sapiens
Biomed Res. Int.
2014
0000
2014
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729520
Ming
SIRT6 promotes COX-2 expressio ...
Homo sapiens, Mus musculus
Cancer Res.
74
5925-5933
2014
-
1
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4
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1
3
3
1
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729679
Kokkonen
Studying SIRT6 regulation usin ...
Homo sapiens
Eur. J. Pharm. Sci.
0
0000
2014
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12
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729849
Wu
Expression and function of SIR ...
Homo sapiens
Int. J. Clin. Exp. Pathol.
7
6504-6513
2014
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1
2
2
1
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730790
Silberman
SIRT6 is required for normal r ...
Homo sapiens
PLoS One
9
e98831
2014
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2
2
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730900
Ringel
Alternate deacylating specific ...
Archaeoglobus fulgidus
Protein Sci.
23
1686-1697
2014
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1
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730024
Feldman
Activation of the protein deac ...
Homo sapiens
J. Biol. Chem.
288
31350-31356
2013
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6
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730101
Jedrusik-Bode
The sirtuin SIRT6 regulates st ...
Mus musculus
J. Cell Sci.
126
5166-5177
2013
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