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Literature summary for 2.3.1.B41 extracted from

  • Zhu, A.Y.; Zhou, Y.; Khan, S.; Deitsch, K.W.; Hao, Q.; Lin, H.
    Plasmodium falciparum Sir2A preferentially hydrolyzes medium and long chain fatty acyl lysine (2012), ACS Chem. Biol., 7, 155-159.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.B41

Application

Application Comment Organism
medicine PfSir2A inhibitors may have therapeutic value in malaria treatment Plasmodium falciparum

Cloned(Commentary)

Cloned (Comment) Organism
-
 Plasmodium falciparum

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, cocrystal of enzyme with [histone H3 peptide]-N6-myristoyl-L-lysine Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
 [histone H3 peptide]-N6-octanoyl-L-lysine pH 8.0, 37°C Plasmodium falciparum
0.008
-
 [histone H3 peptide]-N6-butyryl-L-lysine pH 8.0, 37°C Plasmodium falciparum
0.039
-
 [histone H3 peptide]-N6-acetyl-L-lysine pH 8.0, 37°C Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum Q8IE47
-
-
Plasmodium falciparum isolate 3D7 Q8IE47
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + [histone H3 peptide]-N6-acetyl-L-lysine the enzyme catalyzes the hydrolysis of medium and long chain fatty acyl groups from lysine residues more efficiently than the hydrolysis of acetyl groups Plasmodium falciparum nicotinamide + [histone H3 peptide]-L-lysine + 2'-O-acetyl-ADP-ribose
-
 ?
NAD+ + [histone H3 peptide]-N6-acetyl-L-lysine the enzyme catalyzes the hydrolysis of medium and long chain fatty acyl groups from lysine residues more efficiently than the hydrolysis of acetyl groups Plasmodium falciparum isolate 3D7 nicotinamide + [histone H3 peptide]-L-lysine + 2'-O-acetyl-ADP-ribose
-
 ?
NAD+ + [histone H3 peptide]-N6-butyryl-L-lysine the enzyme catalyzes the hydrolysis of medium and long chain fatty acyl groups from lysine residues more efficiently than the hydrolysis of acetyl groups Plasmodium falciparum nicotinamide + [histone H3 peptide]-L-lysine + 2'-O-butyryl-ADP-ribose
-
 ?
NAD+ + [histone H3 peptide]-N6-butyryl-L-lysine the enzyme catalyzes the hydrolysis of medium and long chain fatty acyl groups from lysine residues more efficiently than the hydrolysis of acetyl groups Plasmodium falciparum isolate 3D7 nicotinamide + [histone H3 peptide]-L-lysine + 2'-O-butyryl-ADP-ribose
-
 ?
NAD+ + [histone H3 peptide]-N6-myristoyl-L-lysine the enzyme catalyzes the hydrolysis of medium and long chain fatty acyl groups from lysine residues more efficiently than the hydrolysis of acetyl groups Plasmodium falciparum nicotinamide + [histone H3 peptide]-L-lysine + 2'-O-myristoyl-ADP-ribose
-
 ?
NAD+ + [histone H3 peptide]-N6-myristoyl-L-lysine the enzyme catalyzes the hydrolysis of medium and long chain fatty acyl groups from lysine residues more efficiently than the hydrolysis of acetyl groups Plasmodium falciparum isolate 3D7 nicotinamide + [histone H3 peptide]-L-lysine + 2'-O-myristoyl-ADP-ribose
-
 ?
NAD+ + [histone H3 peptide]-N6-octanoyl-L-lysine the enzyme catalyzes the hydrolysis of medium and long chain fatty acyl groups from lysine residues more efficiently than the hydrolysis of acetyl groups Plasmodium falciparum nicotinamide + [histone H3 peptide]-L-lysine + 2'-O-octanoyl-ADP-ribose
-
 ?
NAD+ + [histone H3 peptide]-N6-octanoyl-L-lysine the enzyme catalyzes the hydrolysis of medium and long chain fatty acyl groups from lysine residues more efficiently than the hydrolysis of acetyl groups Plasmodium falciparum isolate 3D7 nicotinamide + [histone H3 peptide]-L-lysine + 2'-O-octanoyl-ADP-ribose
-
 ?

Synonyms

Synonyms Comment Organism
Sir2A
-
 Plasmodium falciparum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.001
-
 [histone H3 peptide]-N6-acetyl-L-lysine pH 8.0, 37°C Plasmodium falciparum
0.001
-
 [histone H3 peptide]-N6-butyryl-L-lysine pH 8.0, 37°C Plasmodium falciparum
0.001
-
 [histone H3 peptide]-N6-octanoyl-L-lysine pH 8.0, 37°C Plasmodium falciparum
0.01
-
 [histone H3 peptide]-N6-myristoyl-L-lysine pH 8.0, 37°C Plasmodium falciparum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Plasmodium falciparum

General Information

General Information Comment Organism
physiological function the enzyme regulates the expression of surface antigens to evade the detection by host immune surveillance. The physiological function of PfSir2A in antigen variation may be achieved by removing medium and long chain fatty acyl groups from protein lysine residues Plasmodium falciparum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.026
-
 [histone H3 peptide]-N6-acetyl-L-lysine pH 8.0, 37°C Plasmodium falciparum
0.16
-
 [histone H3 peptide]-N6-butyryl-L-lysine pH 8.0, 37°C Plasmodium falciparum
0.92
-
 [histone H3 peptide]-N6-octanoyl-L-lysine pH 8.0, 37°C Plasmodium falciparum
10
-
 [histone H3 peptide]-N6-myristoyl-L-lysine kcat/KM is above 10/s*mM, pH 8.0, 37°C Plasmodium falciparum