Application | Comment | Organism |
---|---|---|
analysis | simple mass spectrometry-based assay that affords monitoring of FASN activity and its product specificity. Purified FASN is incubated with 13C-labeled malonyl-CoA, acetyl-CoA, and NADPH, at defined time points the reaction mixture is spiked with an internal non-esterified palmitic acid standard and extracted, and the extract is analyzed directly, without vacuum evaporation and chemical derivatization, by direct-infusion high-resolution mass spectrometry in negative ion mode. The assay supports essentially noise-free detection and absolute quantification of de novo synthetized 13C-labeled non-esterified fatty acids | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | Q71SP7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ | - |
Bos taurus | palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O | - |
? |