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Literature summary for 2.3.1.74 extracted from

  • Wani, T.A.; Pandith, S.A.; Gupta, A.P.; Chandra, S.; Sharma, N.; Lattoo, S.K.
    Molecular and functional characterization of two isoforms of chalcone synthase and their expression analysis in relation to flavonoid constituents in Grewia asiatica L. (2017), PLoS ONE, 12, e0179155 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.74

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Grewia asiatica

Organism

Organism UniProt Comment Textmining
Grewia asiatica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione-Sepharose column chromatography Grewia asiatica

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Grewia asiatica
-
leaf
-
 Grewia asiatica
-
petal
-
 Grewia asiatica
-
root highest expression of isoform CHS1 Grewia asiatica
-
stamen
-
 Grewia asiatica
-
stem highest expression of isoform CHS2 Grewia asiatica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 malonyl-CoA + 4-coumaroyl-CoA the reaction has highest efficiency with isoform CHS1. 4-coumaroyl-CoA is the best substrate for isoform CHS1 Grewia asiatica 4 CoA + naringenin chalcone + 3 CO2
-
 ?
malonyl-CoA + acetyl-CoA the reaction has highest efficiency with isoform CHS2 Grewia asiatica ?
-
 ?
malonyl-CoA + butyryl-CoA the reaction has highest efficiency with isoform CHS2 Grewia asiatica ?
-
 ?
malonyl-CoA + hexanoyl-CoA the reaction has highest efficiency with isoform CHS2. Hexanoyl-CoA is the best substrate for isoform CHS2 Grewia asiatica ?
-
 ?
malonyl-CoA + octanoyl-CoA the reaction has highest efficiency with isoform CHS2 Grewia asiatica ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 43000, SDS-PAGE Grewia asiatica

Synonyms

Synonyms Comment Organism
Chs1 isoform Grewia asiatica
Chs2 isoform Grewia asiatica

pI Value

Organism Comment pI Value Maximum pI Value
Grewia asiatica isoform CHS2, calculated from amino acid sequence
-
 7.6
Grewia asiatica isoform CHS1, calculated from amino acid sequence
-
 10.1

Expression

Organism Comment Expression
Grewia asiatica the enzyme expression increases during flowering up