Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-Rosetta (DE3) cells | Polygonum cuspidatum |
Protein Variants | Comment | Organism |
---|---|---|
C198F | the mutant almost completely loses the enzyme activity | Polygonum cuspidatum |
Q82P | the mutant exhibits reduced chalcone-forming activity because its Km value with 4-coumaroyl-CoA as the starter substrate is about 2times higher than that of the wild type enzyme. Additionally, the mutant loses 4-hydroxybenzalacetone-forming activity at pH 7.0-9.0 | Polygonum cuspidatum |
Q82P/C198F | the mutant exhibits benzalacetone synthase activity. The catalytic efficiencies of the mutant for 4-coumaroyl-CoA and malonyl-CoA are lower than that of wild type enzyme | Polygonum cuspidatum |
R105Q | the mutant exhibits benzalacetone synthase activity. The catalytic efficiency of the mutant for 4-coumaroyl-CoA is higher than that of wild type enzyme | Polygonum cuspidatum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0136 | - |
4-coumaroyl-CoA | wild type enzyme, at pH 8.0, temperature not specified in the publication | Polygonum cuspidatum | |
0.0142 | - |
4-coumaroyl-CoA | mutant enzyme R105Q, at pH 8.0, temperature not specified in the publication | Polygonum cuspidatum | |
0.0198 | - |
4-coumaroyl-CoA | mutant enzyme Q82P/C198F, at pH 8.0, temperature not specified in the publication | Polygonum cuspidatum | |
0.0213 | - |
4-coumaroyl-CoA | mutant enzyme Q82P, at pH 8.0, temperature not specified in the publication | Polygonum cuspidatum | |
0.0308 | - |
malonyl-CoA | wild type enzyme, at pH 8.0, temperature not specified in the publication | Polygonum cuspidatum | |
0.0374 | - |
malonyl-CoA | mutant enzyme R105Q, at pH 8.0, temperature not specified in the publication | Polygonum cuspidatum | |
0.0397 | - |
malonyl-CoA | mutant enzyme Q82P/C198F, at pH 8.0, temperature not specified in the publication | Polygonum cuspidatum | |
0.0579 | - |
malonyl-CoA | mutant enzyme Q82P, at pH 8.0, temperature not specified in the publication | Polygonum cuspidatum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 malonyl-CoA + 4-coumaroyl-CoA | Polygonum cuspidatum | - |
4 CoA + naringenin chalcone + 3 CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Polygonum cuspidatum | H9DV62 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Polygonum cuspidatum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 malonyl-CoA + 4-coumaroyl-CoA | - |
Polygonum cuspidatum | 4 CoA + naringenin chalcone + 3 CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Chs1 | - |
Polygonum cuspidatum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
malonyl-CoA | mutant enzyme Q82P, at pH 8.0, temperature not specified in the publication | Polygonum cuspidatum | |
0.05 | - |
4-coumaroyl-CoA | mutant enzyme Q82P, at pH 8.0, temperature not specified in the publication | Polygonum cuspidatum | |
0.055 | - |
malonyl-CoA | mutant enzyme Q82P/C198F, at pH 8.0, temperature not specified in the publication | Polygonum cuspidatum | |
0.063 | - |
malonyl-CoA | mutant enzyme R105Q, at pH 8.0, temperature not specified in the publication | Polygonum cuspidatum | |
0.082 | - |
malonyl-CoA | wild type enzyme, at pH 8.0, temperature not specified in the publication | Polygonum cuspidatum | |
0.085 | - |
4-coumaroyl-CoA | mutant enzyme Q82P/C198F, at pH 8.0, temperature not specified in the publication | Polygonum cuspidatum | |
0.11 | - |
4-coumaroyl-CoA | wild type enzyme, at pH 8.0, temperature not specified in the publication | Polygonum cuspidatum | |
0.178 | - |
4-coumaroyl-CoA | mutant enzyme R105Q, at pH 8.0, temperature not specified in the publication | Polygonum cuspidatum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Polygonum cuspidatum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.66 | - |
malonyl-CoA | mutant enzyme Q82P, at pH 8.0, temperature not specified in the publication | Polygonum cuspidatum | |
1.39 | - |
malonyl-CoA | mutant enzyme Q82P/C198F, at pH 8.0, temperature not specified in the publication | Polygonum cuspidatum | |
1.69 | - |
malonyl-CoA | mutant enzyme R105Q, at pH 8.0, temperature not specified in the publication | Polygonum cuspidatum | |
2.5 | - |
4-coumaroyl-CoA | mutant enzyme Q82P, at pH 8.0, temperature not specified in the publication | Polygonum cuspidatum | |
2.65 | - |
malonyl-CoA | wild type enzyme, at pH 8.0, temperature not specified in the publication | Polygonum cuspidatum | |
4.29 | - |
4-coumaroyl-CoA | mutant enzyme Q82P/C198F, at pH 8.0, temperature not specified in the publication | Polygonum cuspidatum | |
8.21 | - |
4-coumaroyl-CoA | wild type enzyme, at pH 8.0, temperature not specified in the publication | Polygonum cuspidatum | |
12.56 | - |
4-coumaroyl-CoA | mutant enzyme R105Q, at pH 8.0, temperature not specified in the publication | Polygonum cuspidatum |