Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli, strains Nova Blue and BL21(DE3), plasmid pET-28a | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
three-dimensional crystal structure of SSAT with bound bisubstrate inhibitor, solved at 2.3 A resolution, hanging drop method, data and refinement statistics | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N1-spermine-acetyl-CoA | bisubstrate analogue, bisubstrate inhibition patterns determined, linear, competitive inhibition against sperimidine and acetyl-CoA determined | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | no detectable activity for putrescine and cadaverine | Homo sapiens | |
0.0038 | - |
acetyl-CoA | measured at fixed saturating concentration of spermidine | Homo sapiens | |
0.0057 | - |
spermine | measured at fixed saturating concentrations of acetyl-CoA | Homo sapiens | |
0.022 | - |
spermidine | measured at fixed saturating concentrations of acetyl-CoA | Homo sapiens | |
0.107 | - |
1,3-diaminopropane | measured at fixed saturating concentrations of acetyl-CoA | Homo sapiens | |
0.194 | - |
diethylenetriamine | measured at fixed saturating concentrations of acetyl-CoA | Homo sapiens | |
0.196 | - |
ethylenediamine | measured at fixed saturating concentrations of acetyl-CoA | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + spermidine | Homo sapiens | - |
CoA + N1-acetylspermidine | - |
? | |
acetyl-CoA + spermine | Homo sapiens | N1-acetylation of spermidine and spermine by spermidine/spermine acetyltransferase (SSAT) crucial for regulation of the cellular polyamine levels in eukaryotic cells | CoA + N1-acetylspermine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P21673 | hSSAT gene | - |
Purification (Comment) | Organism |
---|---|
gel filtration, recombinant protein, all purification procedures at 4°C | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | cDNA clone of human SSAT | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
kinetic and mechanistic characterization, determination of bisubstrate inhibition patterns, three-dimensional structure of the recombinant human SSAT protein with bound bisubstrate inhibitor, acid/base assisted reaction catalyzed by SSAT involves a ternary complex, proposed on the base of kinetic properties, pH dependence and structure analysis | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 1,3-diaminopropane | - |
Homo sapiens | CoA + N1-acetyl-1,3-diaminopropane | - |
? | |
acetyl-CoA + diethylenetriamine | - |
Homo sapiens | CoA + N1-acetyl-diethylenetriamine | - |
? | |
acetyl-CoA + ethylenediamine | - |
Homo sapiens | CoA + N1-acetyl-ethylenediamine | - |
? | |
acetyl-CoA + spermidine | - |
Homo sapiens | CoA + N1-acetylspermidine | - |
? | |
acetyl-CoA + spermine | N1-acetylation of spermidine and spermine by spermidine/spermine acetyltransferase (SSAT) crucial for regulation of the cellular polyamine levels in eukaryotic cells | Homo sapiens | CoA + N1-acetylspermine | - |
? | |
acetyl-CoA + spermine | N1-acetylation of spermidine and spermine by spermidine/spermine acetyltransferase (SSAT) crucial for regulation of the cellular polyamine levels in eukaryotic cells, chemical and kinetic mechanism for acetyl transfer activity by recombinant human SSAT protein proposed | Homo sapiens | CoA + N1-acetylspermine | - |
? | |
chloroacetyl-CoA + spermine | N1-chloroacetylation of spermine performed by hSSAT protein, pH 7.5 and 2 microM recombinant human SSAT protein at room temperature for one hour, identity of the product confirmed by mass spectrometry | Homo sapiens | N1-spermine-acetyl-CoA + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SSAT | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
determined by pH activity profile | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.7 | 9.1 | activity monitored every 0.3 pH units, bell shaped pH activity profile, depending on ionization state of two groups with apparent pKa values of 7.27 and 8.87 | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
N1-spermine-acetyl-CoA | bisubstrate analogue, linear, competitive inhibition, structure of hSSAT with bound N1-spermine-acetyl-CoA determined | Homo sapiens |